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Summary: Pure suspensions of spores of Eremothecium ashbyii were prepared by filtration through glass wool; the filtrates contained several million spores/ ml. Germinating spores showed antibiotic effects. The efficiency of plating decreased with increasing concentrations of spores but increased when the spores were washed. The results of inactivation by ultraviolet light indicated that the spores were haploid. Inositol deficiency was lethal to the spores. These findings indicate that it should be possible to use E. ashbyii in genetic work, but the lack of knowledge about the life-cycle is a serious obstacle.
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