1887

Abstract

SUMMARY: The incorporation and metabolism of glucose by the blue-green alga is described. Experiments with [C]glucose indicated that this compound contributed up to 46 % of the total dry wt of organism. Respiratory studies with [I-C] and [6-C] showed that the pentose phosphate pathway was the major route of glucose dissimilation. No evidence of alteration in enzyme concentration after growth in the presence of glucose was found in the ten examples of glycolytic and pentose phosphate pathway enzymes examined. Enzymes from both pathways were affected by the end-products. These results are discussed in relation to autotrophic metabolism in blue-green algae.

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1968-12-01
2024-04-20
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References

  1. Axelrod B., Jang R. 1954; Purification and properties of phosphoriboisomerase from alfalfa.. J. biol. Chem. 209:847
    [Google Scholar]
  2. BLOOM B., STETTEN D. 1953; Pathways of glucose catabolism.. J. Am. chem. Soc. 75:5446
    [Google Scholar]
  3. BUCHER T., PFLEIDERER G. 1955; Pyruvate kinase from muscle.. Meth. Enzym. 1:435
    [Google Scholar]
  4. CARR N. G. 1967; Aspects of enzymic control in blue-green algae.. Abstr. 4th Meeting Fed. Europ. Biochem. Soc. Universitetsforlaget, Oslo, p. 89
    [Google Scholar]
  5. Carr N. G., Hallaway H. M. 1965; Reduction of phenolindo-2,6-dichlorophenol in dark and light by the blue-green alga, Anabaena variabilis.. J. gen. Microbiol. 39:335
    [Google Scholar]
  6. Carr N. G.> , Pearce J. 1966; Photoheterotrophism in blue-green algae.. Biochem. J. 99:28P
    [Google Scholar]
  7. Cheung W. Y., Gibbs M. 1966; Dark and photometabolism of sugars by a blue-green alga: Tolypothrix tenuis.. PI. Physiol. Lancaster 41:731
    [Google Scholar]
  8. Dische Z., Borenfreund E. 1951; A new spectrophotometric method for the detection and determination of ketosugars and trioses.. J. biol. Chem. 192583
    [Google Scholar]
  9. FAY P. 1965; Heterotrophy and nitrogen fixation in Chlorogloea fritschii.. J. gen. Microbiol. 39:11
    [Google Scholar]
  10. FEWSON C. A., AL-HAFIDH M., GIBBS M. 1962; Role of aldolase in photosynthesis. I. Enzyme studies with photosynthetic organisms with special reference to blue-green algae.. PI. Physiol. Lancaster 37:402
    [Google Scholar]
  11. FREDRICK J. F. 1954; The synthesis of polysaccharides in algae. V. Kinetics of polysaccharide formation in extractions of Oscillatoria princeps.. Physiologia PI. 7:182
    [Google Scholar]
  12. GIBBS M. 1955; TPN triose phosphate dehydrogenase from plant tissue.. Meth. Enzym. 1:411
    [Google Scholar]
  13. Hamilton W. A., Dawes E. A. 1960; The nature of the diauxic effect with glucose and organic acids in Pseudomonas aeruginosa.. Biochem. J. 76:70P
    [Google Scholar]
  14. Hoare D. S., Moore R. B. 1965; Photoassimilation of organic compounds by autotrophic blue-green algae.. Biochim. biophys. Acta 109:622
    [Google Scholar]
  15. HOARE D. S., HOARE S. L., MOORE R. B. 1967; The photoassimilation of organic compounds by autotrophic blue-green algae.. J. gen. Microbiol. 49:351
    [Google Scholar]
  16. HOLLMAN S. 1964; Non-glycolytic Pathways of Metabolism of Glucose. p. 60 New York and London:: Academic Press.;
    [Google Scholar]
  17. HOOD W., CARR N. G. 1967; A single glyceraldehyde-3-phosphate dehydrogenase active with NAD and NADP in Anabaena variabilis.. Biochim. biophys. Acta 146:309
    [Google Scholar]
  18. HORECKER B. L., SMYRNIOHS P. Z. 1955; 6-Phosphogluconic dehydrogenase.. Meth. Enzym. 1:323
    [Google Scholar]
  19. Ivanov V. I. 1959; Relation between carbohydrate and phosphate metabolism and the formation of a-toxin in B. perfringens.. Vop. med. Khim. Akad. med. Nauk. SSSR. 5:254 in Russian:
    [Google Scholar]
  20. Kelly D. P. 1967; Problems of the autotrophic microorganism.. Sci. Prog. 55:35
    [Google Scholar]
  21. Kindel P. K., Gibbs M. 1963; Distribution of carbon-14 in polysaccharide after photosynthesis in carbon dioxide labelled with carbon-14 by Anacystis nidulans.. Nature, Lond. 200:260
    [Google Scholar]
  22. KIYOHARA T., FUJITA Y., HATTORI A., WATANABE A. 1960; Heterotrophic culture of a blue-green alga, Tolypothrix tenuis.. J. gen. appl. Microbiol. 6:176
    [Google Scholar]
  23. KORNBERG A, HORECKER B. L. 1955; Glucose-6-phosphate dehydrogenase.. Meth. Enzym. 1:323
    [Google Scholar]
  24. KRATZ W. A., MYERS J. 1955a; Photosynthesis and respiration of three blue-green algae, PI.. Physiol. Lancaster 30:275
    [Google Scholar]
  25. KRATZ W. A., MYERS J. 1955b; Nutrition and growth of several blue-green algae.. Am. J. Bot. 42:282
    [Google Scholar]
  26. Lowry O. H., Passonneau J. V. 1964; A comparison of the kinetic properties of phosphofructo- kinase from bacterial, plant and animal sources.. Naunyn-Schmiedebergs Arch. exp. Path. Phar- mak. 248:185
    [Google Scholar]
  27. Pearce J., Carr N. G. 1967a; The metabolism of acetate by the blue-green algae, Anabaena variabilis and Anacystis nidulans.. J. gen. Microbiol. 49:301
    [Google Scholar]
  28. PEARCE J., CARR N. G. 1967b; An incomplete tricarboxylic acid cycle in the blue-green alga Anabaena variabilis.. Biochem. J. 105:45 p.
    [Google Scholar]
  29. Pakoskey A. M., Lesher E. C., Scott D. B. M. 1965; Hexokinase of Escherichia coli, assay of enzyme activity and adaptation to growth in various media.. J. gen. Microbiol. 38:73
    [Google Scholar]
  30. Racker E. 1947; Spectrophotometric measurement of hexokinase and phosphohexokinase activity.. J. biol. Chem. 167:843
    [Google Scholar]
  31. Ramaiach A., Hathaway J. A., Atkinson D. E. 1964; Adenylate as a metabolic regulator.. J. biol. Chem. 239:3619
    [Google Scholar]
  32. Richter G. 1959; Comparison of enzymes of sugar metabolism in two photosynthetic algae; Anacystis nidulans and Chlorella pyrenoidosa.. Naturwissenschaften 46:404
    [Google Scholar]
  33. Roe J. H. 1955; The determination of sugar in blood and spinal fluid with anthrone reagent.. J. biol. Chem. 212:335
    [Google Scholar]
  34. SCOTT D. B. M., CHU E. 1959; The oxidative pathway of carbohydrate metabolism in Escherichia coli.. Biochem. J. 72:426
    [Google Scholar]
  35. SLATER E. C. 1953; Spectrophotometric determination of fructose-1,6-dephosphate, hexosemono- phosphate, adenosine triphosphate and adenosine diphosphate.. Biochem. J. 53:157
    [Google Scholar]
  36. SLEIN M. W., CORI G. T., CORI C. F. 1950; A comparative study of hexokinase from yeast and animal tissues.. J. biol. Chem. 186:763
    [Google Scholar]
  37. Smith A. J., London J., Stanier R. Y. 1967; Biochemical basis of obligate autotrophy in blue- green algae and thiobacilli.. J. Bact. 94:972
    [Google Scholar]
  38. Snyder F., Godfrey P. 1961; Collecting 14COa in a Warburg flask for subsequent scintillation counting.. J. Lipid Res. 2:195
    [Google Scholar]
  39. Taylor C. B., Bailey E. 1967; Activation of liver pyruvate kinase by fructose-1,6-diphosphate.. Biochem. J. 102:32 c
    [Google Scholar]
  40. Van Baalen C. 1965; Aldolase in blue-green algae.. Nature, Lond. 206:193
    [Google Scholar]
  41. Weber G.,, Convery H. J. H.,, Lea M. A., Stamm N. B. 1966; Feedback inhibition of key glycolytic enzymes in liver: action of free fatty acids.. Science, N.Y. 154:1357
    [Google Scholar]
  42. Wildon D. C., Ap Rees T. 1965; Metabolism of glucose-C14 by Anabaena cylindrica.. PI. Physiol., Lancaster 40:332
    [Google Scholar]
  43. WILLARD J. M., GIBBS M. 1967; Purification and characterisation of the fructose diphosphate aldolases from Anacystis nidulans and Saprospira thermalis.. Biochim. biophys. Acta 151:438
    [Google Scholar]
  44. Willard J. M., Schulman M., Gibbs M. 1965; Aldolase in Anacystis nidulans and Rhodo- pseudomonas spheroides.. Nature, Lond. 206:195
    [Google Scholar]
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