1887

Abstract

SUMMARY: required carbon dioxide in substrate concentrations (1·.75%, v/v) to assimilate either nitrate or nitrite at maximum rates in light. The addition of glucose, glycerol, acetate, pyruvate or α-ketoglutarate did not remove the requirement for carbon dioxide. The rates of nitrate and nitrite assimilation in light depended upon the buffer system used. The lowest rates of nitrate assimilation, 1·3 μmoles/hr/mg. chlorophyll, were observed in 0·05 M-phosphate buffer (pH 7·6) and the highest, 13·7 μmoles/hr/mg. chlorophyll, in 0·05 M-Tricine buffer (pH 7·6). Nitrite assimilation was lowest, 7·5 μmoles/hr/mg. chlorophyll, in 0·05 M-phosphate (pH 7·6) while the highest rates, 18·7 μmoles/hr/mg. chlorophyll, were observed in 0·05 M-Tricine (pH 8·6). The low rate of assimilation of nitrate and nitrite in 0·05 M-phosphate buffer (pH 7·6) was increased by diluting the buffer to 0·005 M, at this concentration the rate in phosphate buffer was comparable to that in tris or Tricine buffers at the same pH values. Buffer type had little effect on either nitrate or nitrite assimilation in the dark. There was no evidence with any buffer system used for the evolution of extra oxygen associated with nitrate or nitrite assimilation in the light. These results provide further evidence for the existence of two independent systems of nitrate reduction, one within and the other without the chloroplast. In addition, they indicate that in the enzyme system which fixes carbon dioxide is unlikely to be the rate-limiting step in photosynthesis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-54-3-327
1968-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/54/3/mic-54-3-327.html?itemId=/content/journal/micro/10.1099/00221287-54-3-327&mimeType=html&fmt=ahah

References

  1. BARRETT J., JEFFREY S. W. 1964; Chlorophyllase and formation of an atypical chlorophyllide in marine algae.. PI. Physiol., Lancaster. 39:44
    [Google Scholar]
  2. BONGERS L. H. J. 1958; Kinetic aspects of nitrate reduction.. Neth. J. agric. Sci. 6:79
    [Google Scholar]
  3. CRAIGIE J. S. 1964; Glycerol as a photosynthetic product in . Dunaliella tertiolecta. Can. J. Bot. 41:317
    [Google Scholar]
  4. CRAMER M., MYERS J. 1949; Nitrate reduction and assimilation in Chlorella.. J. gen. Physiol. 32:92
    [Google Scholar]
  5. DAVIS E. A. 1953; Nitrate reduction by Chlorella.. PI. Physiol., Lancaster. 28:539
    [Google Scholar]
  6. Forrester M., Krotkov G., Nelson D. 1966; The effect of oxygen on photosynthesis, photorespiration and respiration in detached leaves. I. Soybean.. PI. Physiol., Lancaster. 41:422
    [Google Scholar]
  7. Gibbs M., Bamberger E. S., Ellyard P. W., Everson G. 1967; Assimilation of carbon dioxideby chloroplast preparations.. In Biochemistry of Chloroplasts. Ed. by Goodwin T. W. Vol. 2 p. 3. London:: Academic Press.;
    [Google Scholar]
  8. Good N. E. 1963; Carbon dioxide and the Hill reaction.. PI. Physiol., Lancaster 38:298
    [Google Scholar]
  9. GOOD N. E. 1965; Interpretations of the carbon dioxide dependence of the Hill reaction.. Can. J. Bot. 43:119
    [Google Scholar]
  10. GRANT B. R. 1967; The action of light on nitrate and nitrite assimilation by the marine chlorophyte Dunaliella tertiolecta (Butcher).. J. gen. Microbiol. 48:379
    [Google Scholar]
  11. HATTORT A. 1962; Light induced reduction of nitrate, nitrite and hydroxyalamine in a blue-green alga, Anabaena cylindrica.. PI. Cell Physiol., Tokyo 3:355
    [Google Scholar]
  12. HOMANN P. H., SCHMID G. H. 1967; Photosynthetic reactions of chloroplasts with unusual structures.. PI. Physiol., Lancaster. 42:1619
    [Google Scholar]
  13. Hulsen W., Prenzel U. 1966; Uber die Aufnahme verschiedener Zucker durch.. Chlorella pyrenoidosa. Z. Naturf. 216:500
    [Google Scholar]
  14. JOY K. W., HAGEMAN R. H. 1966; The purification and properties of nitrite reductase from higher plants and its dependence on ferredoxin.. Biochem. J. 100:263
    [Google Scholar]
  15. KESSLER E. 1953; Ober den Mechanismus der Nitratreduktion von Grunalgen. I. Nitritbildung und Nitritreduktion durch . Ankistrodesmus braunii. Flora, Jena. 76:280
    [Google Scholar]
  16. Kessler E. 1964; Nitrate assimilation in plants.. A. Rev. PI. Physiol. 15:57
    [Google Scholar]
  17. LOSADA M., RAMIREZ J. M., PANEQUE A., DEL. CAMPO F. F. 1965; Light and dark reduction of nitrate in a re-constituted spinach chloroplast system. Biochim. biophys. Acta. 109:86
    [Google Scholar]
  18. MADGWICK J. C. 1966; Chromatographic determination of chlorophylls in algal cultures and phytoplankton. Deep Sea Res. 13:459
    [Google Scholar]
  19. RITENOUR G. L., Joy K. W., BUNNING J., Hageman R. 1967; Intracellular localization of nitrate reductase, nitrite reductase and glutamic acid dehydrogenase in green leaf tissue. PI. Physiol., Lancaster. 42:233
    [Google Scholar]
  20. Rodriguez-Lopez M. 1966; Utilization of sugars by Chlorrella under various conditions.. J. gen. Microbiol. 43:139
    [Google Scholar]
  21. SYRETT P. J. 1955; The assimilation of ammonia and nitrate by nitrogen starved cells of Chlorella vulgaris.I. The assimilation of small quantities of nitrogen.. Physiologia PI. 9:19
    [Google Scholar]
  22. TAYLOR F. J. 1950; Oxidative assimilation of glucose by Scenedesmus quadriculata.. J. exp. Bot. 1:301
    [Google Scholar]
  23. Van Niel C. B., Allen M. B., Wright B. 1953; On the photochemical reductionof nitrate by algae.. Biochim. Biophys. Acta 12:67
    [Google Scholar]
  24. WARBURG O., KRIPPAHL G. 1959; Weiterentwicklung der Manometrischen Methoden. Z. Naturf. :561
    [Google Scholar]
  25. WARBURG O., KRIPPAHL G. 1960; Notwendigkeit der Kohlensaure fur die Chinon und Ferri-cyanid-Reaktion in griinen Grana.. Z.Naturf. 15b:367
    [Google Scholar]
  26. WARBURG O., NEGELEIN E. 1920; Ober die Reduktion der Saltpetersaure in griinen Zellen.. Biochem. Z. 110:66
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-54-3-327
Loading
/content/journal/micro/10.1099/00221287-54-3-327
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error