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SUMMARY: Thymidine kinase (EC 2.7.1.21), an enzyme that catalyses the phosphorylation of thymidine to thymidine-5’-phosphate in the presence of adenosine triphosphate and Mg2+, was not detected in cell-free extracts of Neurospora crassa, Aspergillus nidulans, Saccharomyces cerevisiae or Euglena gracilis. Deoxyribonucleic acid was not specifically labelled in Neurospora crassa grown in the presence of [14C]thymidine, [14C]thymidine monophosphate or [14C]thymidinetr iphosphate. Instead, the isotope was incorporated into both types of nucleic acid in a ratio which approximated the mole ratio of ribonucleic acid to deoxyribonucleic acid. It is considered that the absence of thymidine kinase prevents the specific incorporation of thymidine into deoxyribonucleic acid in intact cells of Neurospora crassa.
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