1887

Abstract

SUMMARY: A relatively rapid and reliable method is described for producing axenic cultures of soil-borne blue-green algae, of the blue-green algal endophytes of certain cycads, and the blue-green endophyte of the angiosperm Endl. It was based on the micro-manipulation of hormogonia and akinetes, from which axenic cultures were propagated. Micromanipulation was done during growth in impure culture on an agar medium. The technique was used to obtain axenic cultures of fifteen soil isolates, the endophytes of the cycads (Linnaeus) Johnson, Lehm., and Bull, and the endophyte of Endl. The soil isolates have been tentatively identified as species of the genera , and

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1968-12-01
2024-12-02
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References

  1. Allen Mary B. 1952; The cultivation of Myxophyceae.. Arch. Mikrobiol. 17:34
    [Google Scholar]
  2. ALLEN, MARY B., ARNON D. I. (1955); Studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm.. Plant Physiol. 30:366
    [Google Scholar]
  3. ALLISON F. E., MORRIS H. J. 1930; Nitrogen fixation by blue-green algae.. Science, N. Y. 71:221
    [Google Scholar]
  4. ALLISON F. E., HOOVER S. R., MORRIS H. J. 1937; Physiological studies with the nitrogen-fixing alga. Nostoc muscorum. Bot. Gaz. 98:433
    [Google Scholar]
  5. BUNT J. S. 1961; Isolation of bacteria-free cultures from hormogone producing blue-green algae.. Nature, Lond. 192:1275
    [Google Scholar]
  6. DE P. K. 1939; The role of blue-green algae in nitrogen fixation in rice fields.. Proc. R. Soc. B, 127:121
    [Google Scholar]
  7. DOUIN R. 1953; Sur la fixation de l·azote libre par les Myxophyces endophytes des Cycadacees.. C. r. Seanc. Acad. aris 236:956
    [Google Scholar]
  8. FOGG G. E. 1942; Studies on nitrogen fixation by blue-green algae. I. Nitrogen fixation by. Anabaena cylindrica Lemm.J. exp. Biol. I9:78
    [Google Scholar]
  9. FOGG G. E. 1944; Growth and heterocyst production in. Anabaena cylindrica Lemm. New Phytol. 43:164
    [Google Scholar]
  10. FOGG G. E. 1951; Studies on nitrogen fixation by blue-green algae. II. Nitrogen fixation by. Mastigocladus laminosus Cohn.J. exp. Bot. 2:117
    [Google Scholar]
  11. FRITSCH F. E. . 1945; The Structure and Reproduction of the Algae, vol. 2.. Cambridge University Press.
    [Google Scholar]
  12. GERLOFF G. C., FITZGERALD G. P., SKOOG F. 1950; The isolation, purification, and culture of blue-green algae.. Am. J. Bot. 37:216
    [Google Scholar]
  13. HENRIKSSON E. 1951; Nitrogen fixation by a bacteria-free, symbiotic Nostoc strain isolated from Collema.. Physiologia PI. iv:542
    [Google Scholar]
  14. HOLM-HANSEN O., GERLOFF G. C., SKOOG F. 1954; Cobalt as an essential element for blue-green algae.. Physiologia PI. 7:665
    [Google Scholar]
  15. JOHNSON L . A. S. 1959; The families of cycads and the Zamiaceae of Australia.. Proc. Linn. Soc. N.S.W. 84:64
    [Google Scholar]
  16. KRAUS M. P. 1966; Preparation of pure blue-green algae.. Nature, Lond. 211:310
    [Google Scholar]
  17. KUMAR H. D. 1963; Effects of radiations on blue-green algae. I. The production and characterization of a strain of . Anacystis nidulans resistant to u-v radiation. Ann. Bot. 27:723
    [Google Scholar]
  18. McDANIEL H. R., MIDDLEBROOK J. B. BOWMAN R. O. 1962; Isolation of pure cultures of algae from contaminated cultures.. Appl. Microbiol. 10:223
    [Google Scholar]
  19. MIDDLEBROOK J. B., BOWMAN R. O. 1964; Preparation of axenic cultures of algae by use of a French press.. Appl. Microbiol. 12:44
    [Google Scholar]
  20. PINTNER I. J., PROVASOLI L. 1958; Artificial cultivation of a red pigmented marine blue-green alga,. Phormidium persicinum. J. gen. Microbiol. 18:190
    [Google Scholar]
  21. PRAMER D. 1957; The influence of physical and chemical factors on the preparation of silica gel media.. Appl. Microbiol. 5:392
    [Google Scholar]
  22. PRESCOTT G. W. 1964; How to Know the Fresh-water Algae.. Iowa: Wm. C. Brown Co. Publishers.
    [Google Scholar]
  23. PRINGSHEIM E. G. 1946; Pure Cultures of Algae.. Cambridge University Press.
    [Google Scholar]
  24. SCHAEDE R. 1951; üt)ber die Blaualgensymbiose von Gunnera.. Planta 39:154
    [Google Scholar]
  25. SCHRAMM J. R. 1914; Some pure culture methods in the algae.. Ann. Mo. bot. Gdn. 1:23
    [Google Scholar]
  26. SINGH R. N., SINGH H. N. 1964; Ultra-violet induced mutants of blue-green algae. I.. Anabaena cycadeae Reinke.Arch. Mikrobiol. 48:109
    [Google Scholar]
  27. SKERMAN V. B. D. 1968; A new type of micromanipulator and microforge.. J. gen. Microbiol. 53:287
    [Google Scholar]
  28. SPRATT, ETHEL R. 1915; The root nodules of the Cycadaceae.. Ann. Bot. 29:619
    [Google Scholar]
  29. STEWART W. D. P. 1962; Fixation of elemental nitrogen by marine blue-green algae.. Ann. Bot. 26:439
    [Google Scholar]
  30. TCHAN Y. T., GOULD J. 1961; Use of antibiotics to purify blue-green algae.. Nature, Lond. 192:1276
    [Google Scholar]
  31. TISCHER R. G. 1965; Pure culture of. Anabaena flos-aquae. Nature, Lond. 205:419
    [Google Scholar]
  32. VENKATARAMAN G. S. 1961; Nitrogen fixation by. Stigonema dendroideum. Indian J. agric. Sci. 31:213
    [Google Scholar]
  33. VENKATARAMAN G. S. 1962; Studies on nitrogen fixation by blue-green algae. III. Nitrogen fixation by. Anabaena azollae. Indian J. agric. Sci. 32:22
    [Google Scholar]
  34. VENKATARAMAN G. S., DUTTA N., NATARAJAN K. V. . 1959; Studies on nitrogen fixation by blue-green algae. I. Nitrogen fixation by . Cylindrospermum sphaerica Prasad. J. Indian bot. Soc. 38:114
    [Google Scholar]
  35. WATANABE A. 1951; Production in cultural solution of some amino acids by the atmospheric nitrogen fixing blue-green algae. Arch. Biochem. Biophys. 34:50
    [Google Scholar]
  36. WATANABE A., KIYOHARA T. 1963; Symbiotic blue-green algae of lichens, liverworts, and cycads. In:. Studies of Microalgae and Photosynthetic Bacteria.Tokyo University Press. 189:
    [Google Scholar]
  37. WINTER G. 1935; über die Assimilation des Luftstickstoffs durch endophytische Blaualgen.. Beitr. Biol. Pfl. 23:295
    [Google Scholar]
  38. WITTMANN W., BERGERSEN F. J., KENNEDY G. S. 1965; The coralloid roots of Macrozamia communis L. Johnson. Aust.. J. biol. Sci. 18:1129
    [Google Scholar]
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