1887

Abstract

SUMMARY: The distribution of [C]-L-cysteine and [C]iodoacetic acid in mycelium and sclerotia of Sacc., and the effect of disodium ethylene-diaminetetraacetic acid (NaEDTA), trans-1, 2-diaminocyclohexane--tetraacetic acid (Chel. C.D.), potassium iodate and phenylthiourea on the formation of sclerotia by were studied. [C]-labelled iodoacetic acid accumulated specifically in the sclerotia, whereas [C]-L-cysteine was equally distributed throughout the mycelium. Accumulation of iodoacetic acid at specific sites was observed even before the formation of the sclerotia. Most of the radioactivity of the fungal mycelium grown on [C]iodoacetic acid was found in the cell-free extract, 93° of the radioactivity of the extract being associated with the ammonium sulphate-precipitated fraction. Na-EDTA, Chel. C.D. and potassium iodate at 10M also induced sclerotial formation. The effect of NaEDTA was eliminated by the addition of 3 × 10M-Cu, but not by Fe, Zn, Co, Mn, Ca nor Mg. Phenylthiourea (10M) initiated sclerotial formation but inhibited further development and melanogenesis. It is suggested that sclerotial formation in is induced by inactivation of a -SH + Cu-containing protein entity which acts as a repressor of sclerotial formation.

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/content/journal/micro/10.1099/00221287-54-2-231
1968-12-01
2021-10-26
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References

  1. BRACHET J. 1964; The role of nucleic acids and sulphydryl groups in morphogenesis. Adv. Morphogenesis 3:247
    [Google Scholar]
  2. >Chet I., Henis Y, & Mitchell R. 1996; The morphogenetic effect of sulphur-containing amino acids, glutathione and iodoacetic acid on Sclerotium rorfsii Sam.. J. gen. Microbiol. 45:541
    [Google Scholar]
  3. Chet I., Henis Y., & Mitchell R. 1967; Chemical composition of hyphal and sclerotial walls of Sclerotium rorfsii Sam. Can.. J. Microbiol 13:137
    [Google Scholar]
  4. FELIX F., & BROUILLET N. 1966; Purification and properties of two peptidases from brewer´s yeast. Biochim.. Biophys. Acta 122:127
    [Google Scholar]
  5. HENIS Y., CHET I., & AVIZOHAR-HERSHENZON Z. 1965; Nutritional and mechanical factors involved in mycelial growth and production of sclerotia by Sclerotium rorfsii in artificial medium and amended soil. Phytopathology 55:87
    [Google Scholar]
  6. JOHAM H. E. 1943; A nutritional study on the fungus Sclerotium rolfsii.. M.Sc. thesis A and M College of Texa;
    [Google Scholar]
  7. JONES G. E. 1964; Effect of chelating agents on the growth of Escherickiu coli in seawater. J. Bact 87:483
    [Google Scholar]
  8. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; P rotein measurement with the Folin reagent.. J. biol. Chem 193:265
    [Google Scholar]
  9. MACLEOD R. A., SMITH L. D. H., GELINAS R. 1966; Metabolic injury to bacteria. I. Effect of freezing and storage on the requirements of Aerobacter aerogenes and Escherichia coli for growth. Can.. J . Microbiol 12:61
    [Google Scholar]
  10. NICKERSON W. J. 1963; Symposium on biochemical basis of morphogenesis in fungi. IV. Molecular basis of form in yeasts.. Bad. Rev 27:305
    [Google Scholar]
  11. NICKERSON W. J., ROMANO A.H. 1952; Enzymatic reduction of cystine by coenzyme I DPNH.. Science, N.Y. 115:676
    [Google Scholar]
  12. SCHRAMM M. 1964; Unmasking of sulphydryl groups in pancreatic a-amylase.. Biochemistry 3:1231
    [Google Scholar]
  13. WHITTAKER J.R. 1966; An analysis of melanogenesis in differentiating Plgment cells of Ascidian embryos.. Devl. Biol. 14:1
    [Google Scholar]
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