1887

Abstract

SUMMARY: Cell walls were prepared from various species of the genus which are resistant to ethylenediaminetetra-acetic acid (EDTA). The cell walls were analysed and comparisons made with the walls of EDTA-sensitive pseudomonads. The walls had none of the structural features which appeared to characterize EDTA-sensitive pseudomonads. Lipopolysaccharide was not extracted from the walls of resistant organisms by EDTA at pH 9.2. The wall of contained almost no lipid or protein but consisted mainly of glycosaminopeptide and material which resembled a teichoic acid. It is proposed that this organism be removed from the genus The walls of and had compositions broadly characteristic of Gram-negative bacteria. The four species are not obviously related. Glycolipids were present in the walls of and An ornithine-containing lipid was isolated from and partly characterized. A small amount of this lipid was also present in The wall of was distinctive in its wide range of monosaccharide components, including an unidentified neutral sugar of high mobility on paper chromatograms.

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1968-12-01
2024-04-23
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References

  1. ALLEN R. J. L. 1940; The estimation of phosphorus. Biochem. J. 34:858
    [Google Scholar]
  2. ASBELL M. A., EAGON R. G. 1966a; The role of multivalent cations in the organization and structure of bacterial cell walls.. Biochem. biophys. Res. Commun. 22:664
    [Google Scholar]
  3. ASBELL M. A., EAGON R. G. 1966b; Role of multivalent cations in the organization, structure, and assembly of the cell wall of Pseudomonas aeruginosa.. J. Bad. 92:380
    [Google Scholar]
  4. BADDELEY J., BUCHANAN J. G., CARSS B. 1957; A method for the identification of pentitols and hexitols.. J. chem. Soc. p. 4138:
    [Google Scholar]
  5. BADDILEY J., BUCHANAN J. G., HANDSCHUMACHER R. E., PRESCOTT J. F. 1956; Biosynthesis of purine nucleotides. I. Preparation of IV-glycylglycosylamines.. J. chem. Soc. p. 2818:
    [Google Scholar]
  6. BARTLETT G. R. 1959; Phosphorus assay in column chromatography.. J. biol. Chem. 234:466
    [Google Scholar]
  7. BOAS N. F. 1953; Method for the determination of hexosamines in tissues.. J. biol. Chem. 204:553
    [Google Scholar]
  8. BRENNER M., NIEDERWIESER A., PATAKI G. 1961; Dunnschicht-chromatographie von amino- saurederivaten auf kieselgel G. N-(2,4-dinitrophenyl)-aminosäuren und 3-phenyl-2-thiohydan- toine.. Experientia 17:145
    [Google Scholar]
  9. BRENNER M., NIEDERWIESER A., PATAKI G. 1964; Characterization of protein hydrolysates and amino acids in biological materials by thin-layer chromatography.. In New Biochemical Separations. Ed. by James A. T., Morris L. J. p. 123 London:: Van Nostrand Co. Ltd.;
    [Google Scholar]
  10. BRYSON J. L., MITCHELL T. J. 1951; Improved spraying reagents for the detection of sugars on paper chromatograms.. Nature, Lond. 167:864
    [Google Scholar]
  11. CLARKE K., GRAY G. W., REAVELEY D. A. 1967; The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. The insoluble residue and material from the aqueous layers.. Biochem. J. 105:759
    [Google Scholar]
  12. Courtade S., Marinetti G. V., Stotz E. 1967; The structure and abundance of rat tissue cardiolipins. Biochim.. biophys Acta 137:121
    [Google Scholar]
  13. Cynkin M. A., Ashwell G. 1960; Estimation of 3-deoxy sugars by means of the malonaldehyde- thiobarbituric acid reaction.. Nature, Lond. 186:155
    [Google Scholar]
  14. Dawson R. M. C. 1960; A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples.. Biochem. J. 75:45
    [Google Scholar]
  15. DE HAAS G. H., BONSEN P. P. M., VAN DEENEN L. L.M. 1966; Studies on cardiolipin. III. Structural identity of ox-heart cardiolipin and synthetic diphosphatidyl glycerol. Biochim. biophys.. Acta 116:114
    [Google Scholar]
  16. DE LEY J. 1964; Pseudomonas and related genera. A.. Rev. Microbiol. 18:17
    [Google Scholar]
  17. De Ley J., Park I. W., Tijtgat R., Van Ermengem J. 1966; DNA homology and taxonomy of Pseudomonas and Xanthomonas.. J. gen. Microbiol. 42:43
    [Google Scholar]
  18. DEPINTO J. A. 1967; Ornithine-containing lipid in Rhodospirillum rubrum.. Biochim. biophys. Acta 144:113
    [Google Scholar]
  19. Dische Z., Shettles L. B. 1948; A specific color reaction of methylpentoses and a spectrophoto- metric micromethod for their determination.. J. biol. Chem. 175:595
    [Google Scholar]
  20. Dittmer J. C., Lester R. L. 1964; A simple specific spray for the detection of phospholipids on thin-layer chromatograms.. J. Lipid Res. 5:126
    [Google Scholar]
  21. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances.. Analyt. Chem. 28:350
    [Google Scholar]
  22. DUTHIE A. H., PATTON S. 1965; Purification of phospholipids recovered from thin-layer chromatograms for infrared spectral analysis.. J. Lipid Res. 6:320
    [Google Scholar]
  23. EAGON R. G., CARSON K. J. 1965; Lysis of cells walls and intact cells of Pseudomonas aeruginosa by ethylenediamine tetraacetic acid and by lysozyme.. Can. J. Microbiol. 11:193
    [Google Scholar]
  24. EDWARD J. T., WALDRON D. M. 1952; The detection of deoxy-sugars, glycals, and methyl pentoses in paper partition chromatography.. J. chem. Soc. p. 3631:
    [Google Scholar]
  25. ELLWOOD D. C. 1966; The distribution of 3-deoxy-2-oxo-sugar acids in the cell walls of Gram- negative bacteria.. Biochem. J. 99:55
    [Google Scholar]
  26. Fischer F. G., Nebel H. J. 1955; Qualitative and quantitative estimation of glucosamine and galactosamine by the method of paper chromatography.. Hoppe-Seyl. Z. 302:10
    [Google Scholar]
  27. GILMAN J. P. 1953; Studies on certain species of bacteria assigned to the genus Chromobacterium.. J. Bact. 65:48
    [Google Scholar]
  28. Goldschmidt M. C., Wyss O. 1967; The role of tris in EDTA toxicity and lysozyme lysis.. J. gen. Microbiol. 47:421
    [Google Scholar]
  29. Gorchein A. 1964; Ornithine in Rhodopseudomonas spheroides.. Biochim. biophys. Acta 84:356
    [Google Scholar]
  30. Gray G. W., Wilkinson S. G. 1965a; The action of ethylenediaminetetra-acetic acid on Pseudomonas aeruginosa.. J. appl. Bact. 28:153
    [Google Scholar]
  31. Gray G. W., Wilkinson S. G. 1965b; The effect of ethylenediaminetetra-acetic acid on the cell walls of some Gram-negative bacteria.. J. gen. Microbiol. 39:385
    [Google Scholar]
  32. Hanes C.S., Isherwood F.A. 1949; Separation of the phosphoric esters on the filter paper chromatogram.. Nature, Lond. 164:1107
    [Google Scholar]
  33. IKAWA M. 1967; Bacterial phosphatides and natural relationships.. Bact. Rev. 31:54
    [Google Scholar]
  34. Ikawa M., Morrow J. W., Harney S. J. 1966; Paper chromatographic system for the identification of glycerol in bacterial cell walls.. J. Bact. 92:812
    [Google Scholar]
  35. KARKKAINE J., Lehtonen A., Nikkari T. 1965; Determination of glucosamine and galactosamine by gas chromatography.. J. Chromat. 20:457
    [Google Scholar]
  36. KATES M. 1964; Bacterial lipids.. Adv. Lipid Res. 2:17
    [Google Scholar]
  37. LAÉELLME M.-A., LANÉELLGE G., ASSELINEAJU J. 1963; Sur la présence d’omithine dans des lipides bactériens.. Biochim. biophys Acta 70:99
    [Google Scholar]
  38. Leive L. 1965; Release of lipopolysaccharide by EDTA treatment of E. coli.. Biochim. biophys. Res. Commun. 21:290
    [Google Scholar]
  39. MACGREGOR D. R., ELLIKER P. R. 1958; A comparison of some properties of strains of Pseudomonas aeruginosa sensitive and resistant to quaternary ammonium compounds.. Can. J. Microbiol. 4:499
    [Google Scholar]
  40. Maruo B., Benson A. A. 1959; Cyclic glycerophosphate formation from the glycerolphospha- tides.. J. biol. Chem. 234:254
    [Google Scholar]
  41. MORRISON W. R., SMITH L. M. 1964; Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol.. J. Lipid Res. 5:600
    [Google Scholar]
  42. MUKERJEE H., RAM J. S. 1964; Paper chromatographic separation of glucosamine and galactosamine.. Analyt. Biochem. 8:393
    [Google Scholar]
  43. NICHOLS B. W. 1964; Separation of plant phospholipids and glycolipids.. In New Biochemical Separations. Ed. by James A. T., Morris L. J. p. 321: London: Van Nostrand Co. Ltd.;
    [Google Scholar]
  44. OSBORN M. J. 1963; Studies on the Gram-negative cell wall. I. Evidence for the role of 2-keto-3- deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium.. Proc. natn. Acad. Sci., U.S.A. 50:499
    [Google Scholar]
  45. Pataki G. 1967; Thin-layer chromatography of amino acids.. Chromat. Rev. 9:23
    [Google Scholar]
  46. Perkins H. R., Cummins C. S. 1964; Ornithine and 2,4-diaminobutyric acid as components of the cell walls of plant pathogenic Corynebacteria.. Nature, Lond. 201:1105
    [Google Scholar]
  47. RADHAKRISHNAMURTHY B., DALFERES E. R., BERENSON G.S. 1966; Determination of hexos- amines by gas-liquid chromatography.. Analyt. Biochem. 17:545
    [Google Scholar]
  48. ROUSER G., KRITCHEVSKY G., HELLER D., LIEBER E. 1963; Lipid composition of beef brain, beef liver, and the sea anemone: two approaches to quantitative fractionation of complex lipid mixtures.. J. Amer. Oil Chem. Soc. 40:425
    [Google Scholar]
  49. Shwely J. M., Hartsell S. E. 1964; Bacteriolysis of the pseudomonads.. I. Agents potentiating lysis. Can. J. Microbiol. 10:905
    [Google Scholar]
  50. SKIPSKI V. P., BARCLAY M., REICHMAN E. S., GOOD J. J. 1967; Separation of acidic phospholipids by one-dimensional thin-layer chromatography.. Biochim. biophys. Acta 137:80
    [Google Scholar]
  51. Skipski V. P., Peterson R., Sanders J., Barclay M. 1963; Thin-layer chromatography of phospholipids using silica gel without calcium sulphate binder.. J. Lipid Res. 4:227
    [Google Scholar]
  52. SKIPSKI V. P., SMOLOWE A. F., SULLIVAN R. C., BARCLAY M. 1965; Separation of lipid classes by thin-layer chromatography.. Biochim. biophys. Acta 106:386
    [Google Scholar]
  53. SNEATH P. H. A. 1960; A study of the bacterial genus Chromobacterium.. Iowa St. J. Sci. 34:429
    [Google Scholar]
  54. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study.. J. gen. Microbiol. 43:159
    [Google Scholar]
  55. STOFFYN P. J., JEANLOZ R. W. 1954; Hyaluronic acid and related substances. XII. Identification of amino sugars by paper chromatography.. Arch. Biochem. 52:373
    [Google Scholar]
  56. Sweeley C. C., Bentley R., Maktta M., Wells W. W. 1963; Gas-liquid chromatography of trimethyl silyl derivatives of sugars and related substances.. J. Am. chem. Soc. 85:2497
    [Google Scholar]
  57. TOBIE W. C. 1945; A proposed biochemical basis for the genus Pseudomonas.. J. Bact. 49:459
    [Google Scholar]
  58. TREVELYAN W. E., PROCTOR D. P., HARRISON J. S. 1950; Detection of sugars on paper chromatograms.. Nature, Lond. 166:444
    [Google Scholar]
  59. UMBREIT W. W., BURRIS R. H., STAUFFER J. F. 1957; Manometric Techniques. , 3rd ed.,. p. 274: Minneapolis:: Burgess Publishing Co.;
    [Google Scholar]
  60. Vincent J. M., Humphrey B., North R. J. 1962; Some features of the fine structure and chemical composition of Rhizobium trifiolii.. J. gen. Microbiol. 29:551
    [Google Scholar]
  61. Voss J. G. 1967; Effects of organic cations on the Gram-negative cell wall and their bactericidal activity with ethylenediamine-tetra-acetate and surface active agents.. J. gen. Microbiol. 48:391
    [Google Scholar]
  62. WAGNER H., Hö RHAMMER L., WOLFF P. 1961; Dü schichtchromatographie von phosphatiden und glykolipiden.. Biochem. Z. 334:175
    [Google Scholar]
  63. WARREN L. 1960; Thiobarbituric acid spray reagent for deoxy sugars and sialic acids.. Nature, Lond. 186:237
    [Google Scholar]
  64. WESTPHAL O., LÜ DERITZ O. 1960; 3,6-Dideoxy-hexosen - Chemie und Biologie.. Angew. Chem. 72:881
    [Google Scholar]
  65. WHEELDON L. W., COLLINS F. D. 1957; Studies on phospholipids. I. The determination of amino nitrogen in unhydrolysed phospholipids.. Biochem. J. 66:435
    [Google Scholar]
  66. Wilkinson S. G. 1967; The sensitivity of pseudomonads to ethylene-diaminetetra-acetic acid.. J. gen. Microbiol. 47:67
    [Google Scholar]
  67. Wilkinson S. G. 1968; Glycolipids containing glucose and uronic acids in Pseudomonas species.. Biochim. biophys. Acta 152:227
    [Google Scholar]
  68. Wolin M. J. 1966; Lysis of Vibrio succinogenes by ethylenediamine-tetraacetic acid or lysozyme.. J. Bact. 91:1781
    [Google Scholar]
  69. WORK E. 1957; Reaction of ninhydrin in acid solution with straight-chain amino acids containing two amino groups and its application to the estimation of a e-diaminopimelic acid.. Biochem. J. 67:416
    [Google Scholar]
  70. Young D. A. B. 1964; Use of butyl acetate as a solvent for the extraction of trichloroacetic acid.. Biochim. biophys. Acta 90:178
    [Google Scholar]
  71. ZIMINSKI T., BOROWSKI E. 1966; A new spray reagent replacing sulphuric acid in thin-layer chromatography.. J. Chromat. 23:480
    [Google Scholar]
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