Aflatoxin B Binding and Toxic Effects on Free

Abstract

SUMMARY: A study was made of the binding of aflatoxin B and its toxic effects on NRRL B-1368. After a 12 hr incubation period, 5 μg. aflatoxin Bm1. inhibited growth of the test organism 95 %. Viable organisms decreased from 3·8 ⨯ 10/ml. in the controls to 5·0 ⨯ 10/ml. in a growth medium containing 50 μg. aflatoxin B/ml., after 4 hr. of incubation. Viability was not significantly decreased, however, when the organisms were incubated with the same concentration of toxin either under nitrogen, or at 5°, or in the presence of bacteriostatic concentrations of tetracycline. Binding experiments showed that 4·0 ⨯ 10 organisms/ml. took up approximately 7.0 μg. aflatoxin B/ml. After five aqueous washes of the organisms, 20-25% of the toxin remained bound to them. The tightly bound toxin was removed by ultrasonic treatment of the organisms and chloroform extraction of the macerate. Removal of cell walls by lysozyme following toxin uptake released 23% of the initially bound toxin, and osmotic rupture of the protoplasts an additional 7%. Toxin was also taken up by intact and by ruptured protoplasts. Removal of nucleic acids from the membranes did not alter their capacity to bind aflatoxin B.

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/content/journal/micro/10.1099/00221287-54-2-185
1968-12-01
2024-03-28
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References

  1. BERRAH G., KONETZKA W. A. 1962; Selective and reversible inhibition of the synthesis of bacterial deoxyribonucleic acid by phenethyl alcohol. J. Bact. 83:738
    [Google Scholar]
  2. BLACK H. S., JIRGENSONS B. 1967; Interaction of aflatoxin with histones and DNA.. PI. Physiol. Lancaster 42:73I
    [Google Scholar]
  3. BURMEISTER H. R., HESSELTINE C. W. 1966; Survey of the sensitivity of microorganisms to aflatoxin.. Appl. Microbiol I4:403
    [Google Scholar]
  4. BURMEISTER H. R., HESSELTINE C. W. 1967; Aflatoxin sensitivities of an L form and. Bacillus megaterium. Bact. Proc. 1967; I7:
    [Google Scholar]
  5. BURTON K. 1956; A study of the conditions and mechanism of diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.. Biochem. J 62:315
    [Google Scholar]
  6. CIEGLER A., LILIEHOJ E. B., PETERSON R. E., HALL H. H. 1966; Microbial detoxification of aflatoxin.. Appl. Microbiol. 14:934
    [Google Scholar]
  7. CLIFFORD J. I., REES K. R. 1966; Aflatoxin: a site of action in the rat liver cell.. Nature, Lond 209:3I2
    [Google Scholar]
  8. CLIFFORD J. I., REES K. R. 1967; The interaction of aflatoxins with purines and purine nucleosides.. Biochem. J 103:467
    [Google Scholar]
  9. HAYNES W. C., WICKERHAM L. J. 1955; Maintenance of cultures of industrially important microorganisms. Appl. Microbiol. 3:36I
    [Google Scholar]
  10. JACOB F., CUZIN F. 1963; On the regulation of DNA replication in bacteria.. Cold Spring Harb. Symp. quant. Biol. 28:329
    [Google Scholar]
  11. LEGATOR M. S., ZUFFANTE S. M., HARP A. R. 1965; Aflatoxin: effect on cultured heteroploid human embryonic lung cells.. Nature, Lond. 208:345
    [Google Scholar]
  12. LILIEHOJ E. B., CIEGLER A. 1967; Inhibition of deoxyribonucleic acid synthesis in Flavobacterium aurantiacum. by aflatoxin BI J. Bact. 94:787
    [Google Scholar]
  13. LILLEHOJ E. B., CIEGLER A., HALL H. H. 1967; Aflatoxin Biuptake by Flavobacterium aurantiacum. and resulting toxic effects J.BACT 93:464
    [Google Scholar]
  14. MEJBAUM W. 1939; Ober die Bestimmung kleiner Pentosemenger, insbesondere in Derivaten der Adenylsaiire.. Hoppe-Seylerx0027;s Z. physiol. Chem. 258:117
    [Google Scholar]
  15. NABNEY J., NESBITT B. F. 1965; A spectrophotometric method for determining the aflatoxins.. Analyst., Lond. 90:I55
    [Google Scholar]
  16. ROBERTS R. B., MCQUILLEN K. 1959; Biosynthetic aspects of metabolism.. A. Rev. Microbiol. 13:1
    [Google Scholar]
  17. SHOTWELL O. L., HESSELTINE C. W., STUBBLEFIELD R. D., SORENSON R. D. 1966; Production of aflatoxin on rice.. Appl. Microbiol. 14:425
    [Google Scholar]
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