1887

Abstract

SUMMARY

T-mycoplasmas were isolated by a ‘colour-change’ technique, involving the metabolism of urea, from 16 of 49 slaughtered cows (33%). They were encountered more often in the urethra and bladder than in the vagina. Isolations were made usually within 2 days of incubation but occasionally 5 days was required. T-mycoplasmas were isolated in medium without inhibitors from urethral scrapings of a cow and from seminal fluid of a bull. On solid medium, bovine T-mycoplasmas produced colonies of 5-12μ which were similar to those produced by a T-mycoplasma from the human urogenital tract. In liquid medium, the growth cycle of bovine T-mycoplasmas was similar to that of human T-mycoplasmas, being more rapid than that of large colony-forming (classical) mycoplasmas. The presence of serum in the medium was necessary for growth. T-mycoplasmas of bovine origin were affected in a similar way to those of human origin when subjected to ether, heat, freezing and thawing, freeze-drying, antibiotics and thallium acetate; they were more sensitive to erythromycin and thallium acetate than some classical mycoplasmas. The size of the minimal reproductive forms of bovine and human T-mycoplasmas and a classical mycoplasma, , was similar as judged by filtration experiments. Electron microscopy of bovine and human T-mycoplasmas revealed cells which varied in size from 80 mμ, to 420 mμ, and which were bounded by a triple-layered membrane, features similar to known classical mycoplasmas.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-54-1-33
1968-11-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/54/1/mic-54-1-33.html?itemId=/content/journal/micro/10.1099/00221287-54-1-33&mimeType=html&fmt=ahah

References

  1. ANDERSON D. R., BARILE M. F. 1965; Ultrastructure of Mycoplasma hominis.. J. Bad. 90:180
    [Google Scholar]
  2. DEL GIUDICE R. A., PAVIA R. 1964; Hemadsorption by Mycoplasma pneumoniae and its inhibition with sera from patients with primary atypical pneumonia. Bact. Proc. 71:
    [Google Scholar]
  3. FORD D. K. 1967; Relationships between Mycoplasma and the etiology of nongonococcal urethritis and Reiter's syndrome.. Ann. N. Y. Acad. Sci. 143:501
    [Google Scholar]
  4. FORD D. K., DUVERNET M. 1963; Genital strains of human pleuropneumonia-like organisms. Br. J. yen. Dis. 39:18
    [Google Scholar]
  5. FORD D. K., & MACDONALD J. 1963; Morphology of human genital “T-strain” pleuropneumonia like organisms. J. Bact. 85:649
    [Google Scholar]
  6. KLIENEBERGER-NOBEL E. 1962; Pleuropneumonia-Like Organisms (PPLO). Mycoplasmataceae p. 61: London: Academic Press Inc.;
    [Google Scholar]
  7. KUNDSIN R. B., DRISCOLL S. G., MING P.-M. L. 1967; Strain of Mycoplasma associated with human reproductive failure. Science N. Y. 157:1573
    [Google Scholar]
  8. LEACH R. H. 1967; Comparative studies of mycoplasma of bovine origin. Ann. N. Y. Acad. Sci. 143:305
    [Google Scholar]
  9. MANCHEE R. J., TAYLOR-ROBINSON D. 1968; Haemadsorption and haemagglutination by mycoplasmas. J. gen. Microbiol. 50:465
    [Google Scholar]
  10. MOLLENHAUER H. H. 1964; Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39:111
    [Google Scholar]
  11. PURCELL R. H., TAYLOR-ROBINSON D., WONG D., CHANOCK R. M. 1966; A colour test for the measurement of antibody to T-strain mycoplasmas. J. Bact. 92:6
    [Google Scholar]
  12. PURCELL R. H., WONG D., CHANOCK R. M., TAYLOR-ROBINSON D., CANCHOLA J., VALDESUSO J. 1967; Significance of antibody to Mycoplasma as measured by metabolic-inhibition techniques.. Ann. N. Y. Acad. Sci. 143:664
    [Google Scholar]
  13. SHEPARD M. C. 1954; The recovery of pleuropneumonia-like organisms from Negro men with and without nongonococcal urethritis. Am. J. Syph. Gonor. ven. Dis. 38:113
    [Google Scholar]
  14. SHEPARD M. C. 1966; Human mycoplasma infections. Health Lab. Sci. 3:163
    [Google Scholar]
  15. SHEPARD M. C. 1967; Cultivation and properties of T-strains of Mycoplasma associated with nongonococcal urethritis. Ann. N. Y. Acad. Sci. 143:505
    [Google Scholar]
  16. Shepard M. C., Lunceford C. D. 1967; Occurrence of urease in T-strains of Mycoplasma. J. Bact. 93:1513
    [Google Scholar]
  17. SHEPARD M. C., LUNCEFORD C. D., BAKER R. S. 1966; T-strain mycoplasma: selective inhibition by erythromycin in vitro. Br. J. ven. Dis. 42:21
    [Google Scholar]
  18. Somerson N. L., Purcell R. H., Taylor-Robinson D., Chanock R. M. 1965; Hemolysin of Mycoplasma pneumoniae. J. Bact. 89:813
    [Google Scholar]
  19. TAYLOR-ROBINSON D. 1967; Mycoplasmas of various hosts and their antibiotic sensitivities. Postgrad. med. J. 43: (Suppl.) 100
    [Google Scholar]
  20. TAYLOR-ROBINSON D., PURCELL R. H. 1966; Mycoplasmas of the human urogenital tract and oropharynx and their possible role in disease: a review with some recent observations. Proc. roy. Soc. Med. 59:1112
    [Google Scholar]
  21. TAYLOR-ROBINSON D., HAIG D. A., WILLIAMS M. H. 1967; Bovine T-strain Mycoplasma. Ann. N. Y. Acad. Sci. 143:517
    [Google Scholar]
  22. WILLIAMS M. H. 1967; Electron microscopy of T-strains. Ann. N. Y. Acad. Sci. 143:397
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-54-1-33
Loading
/content/journal/micro/10.1099/00221287-54-1-33
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error