1887

Abstract

SUMMARY: A method for the preparation of protoplasts of is described. The protoplasts obtained were very active metabolically and lysates of them were rich in polyribosomes. Methods for the detection of polyribosomes and of nascent peptides are detailed.

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/content/journal/micro/10.1099/00221287-53-3-425
1968-10-01
2024-12-01
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References

  1. Cundliffe E. 1967a; Chlortetracycline and polyribosomes.. J. gen. Microbiol 48:vi
    [Google Scholar]
  2. Cundliffe E. 1967b; Antibiotics and polyribosomes: chlortetracycline and polyribosomes of Bacillus megaterium.. Mol. Pharmacol 3:401
    [Google Scholar]
  3. Cundliffe E. 1967c; Polyribosomes in protoplasts of Bacillus megaterium.. J. gen. Microbiol 46:viii
    [Google Scholar]
  4. Cundliffe E. 1967d Ph.D. Dissertation: University of Cambridge, England.;
  5. Cundliffe E., McQuillen K. 1967a; Peptide bond formation in bacteria.. J. gen. Microbiol 50:x
    [Google Scholar]
  6. Cundliffe E., McQuillen K. 1967b; Bacterial protein synthesis: The effects of antibiotics.. J. molec. Biol 30:137
    [Google Scholar]
  7. Flessel C.P., Ralph P., Rich A. 1967; Polyribosomes of growing bacteria.. Science, N.Y 158:658
    [Google Scholar]
  8. McQuillen K. 1960 The Bacteria Ed. by Gunsalus U. C., Stanier R. Y. 1249 New York and London: Academic Press.;
    [Google Scholar]
  9. McQuillen K., Roberts R. B. 1954; The utilization of acetate for synthesis in Escherichia coli.. J. biol. Chem 207:81
    [Google Scholar]
  10. Mangiarotti G., Schlessinger D. 1967; Polyribosome metabolism in Escherichia coli.. J. molec. Biol 20:123
    [Google Scholar]
  11. Schaechter M., Previc E. P., Gillespie M. E. 1965; Messenger RNA and polyribosomes in Bacillus megaterium.. J. molec. Biol 12:119
    [Google Scholar]
  12. Yudkin M. D. 1963; The effect of penicillin, novobiocin, streptomycin and vancomycin on membrane synthesis by protoplasts of Bacillus megaterium.. Biochem. J 89:290
    [Google Scholar]
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