1887

Abstract

SUMMARY: L-Alanine-initiated germination of spores of was potentiated by the following structural analogues of alanine: -carbamyl-D-serine (OCDS), D-cycloserine (DCS), β-alanylhydroxamic acid (βAHA) and glycyl hydroxamic acid (GHA); but not by D-α-alanyl-hydroxamic acid (DAHA). Potentiation of germination resulted from inhibition of alanine racemase in the spores with consequent suppression of the formation of D-alanine, an inhibitor of L-alanine-initiated germination. OCDS was the most effective potentiator of germination and inhibitor of racemase. βAHA and GHA were more effective potentiators of germination than could be explained solely by their weak inhibition of the racemase. The extra effectiveness was associated with slow binding of the analogues to the spores, and possibly also with formation of hydroxylamine which also inhibited alanine racemase and potentiated L-alanine-initiated germination. Germination initiated by ribosides and amino acids other than L-alanine was not strongly potentiated by OCDS, arguing against a role for L-alanine as an intermediate. However, germination initiated by adenosine+D-alanine was strongly inhibited by OCDS, which argues for the role of D-alanine being to supply the L-isomer by racemization. Antibacterial activities of the analogues did not mirror their activities as potentiators of germination.

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1968-10-01
2023-02-08
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References

  1. Church B. D., Halvorson H. O. 1954; Studies on spore germination: its independence from alanine racemase activity.. J. Bact 68:393
    [Google Scholar]
  2. Edwards J. C., Busta F. F., Speck M. L. 1965; Thermal inactivation characteristics of Bacillus subtilis spores at ultra high temperatures.. Appl. Microbiol 13:851
    [Google Scholar]
  3. Fey G., Gould G. W., Hitchins A. D. 1964; Identification of d-alanine as the auto-inhibitor of germination of Bacillus globigii spores.. J. gen. Microbiol 35:229
    [Google Scholar]
  4. Freeze E., Cashel M. 1965; Initial stages of germination.. Spores ed. by Campbell L. L., Halvorson H. O. 3114 Ann Arbor, Michigan: American Society for Microbiology.;
    [Google Scholar]
  5. Gale G. R., Hawkins J. E. 1965; Antimycobacterial properties of glycyl hydroxamic acid and β-alanyl hydroxamic acid.. Am. Rev. resp. Diseases 92:642
    [Google Scholar]
  6. Gale G. R., Hynes J. B. 1966; Further studies of the antimycobacterial agents glycyl hydroxamic acid and β-alanyl hydroxamic acid.. Can. J. Microbiol 12:73
    [Google Scholar]
  7. Gould G. W. 1966; Stimulation of l-alanine-induced germination of Bacillus cereus spores by d-cycloserine and O-carbamyl-d-serine.. J. Bad 92:1261
    [Google Scholar]
  8. Hermier J., Rousseau M. 1967; La germination de la spore de Bacillus subtilis. IV. Role des acides amines dans la perte de refringence de la spore.. Annls. Inst. Pasteur, Paris 113:327
    [Google Scholar]
  9. Hitchins A. D., Gould G. W., Hurst A. 1963; The swelling of bacterial spores during germination and outgrowth.. J. gen. Microbiol 30:445
    [Google Scholar]
  10. Hills G. M. 1949; Chemical factors in the germination of spore-bearing aerobes. Effect of amino acids on the germination of Bacillus anthracis, with some observations on the relation of optical form to biological activity.. Biochem. J 45:363
    [Google Scholar]
  11. Hills G. M. 1950; Chemical factors in the germination of spore-bearing aerobes. Observations on the influence of species, strain and conditions of growth.. J. gen. Microbiol 4:38
    [Google Scholar]
  12. Krask B. J. 1961; l-Alanine dehydrogenase in spores.. Discussion in Spores ed. by Halvorson H. O. 289 Minneapolis, Minnesota: Burgess Publishing Co.;
    [Google Scholar]
  13. Krask B. J., Fulk G. E. 1966; Enzyme locus: a factor in Bacillus cereus spore germination independence from l- and d-cysteine desulphydrase activities.. Bact. Proc32
    [Google Scholar]
  14. Lawrence N. L. 1955; The cleavage of adenosine by spores of Bacillus cereus.. J. Bact 70:577
    [Google Scholar]
  15. Lynch J. L., Neuhaus F. C. 1966; On the mechanism of action of the antibiotic O-carbamyl-d-serine in Streptococcus faecalis.. J. Bact 91:449
    [Google Scholar]
  16. Neuhaus F. C. 1967; d-Cycloserine and O-carbamyl-d-serine.. Antibiotics: Mechanism of Action Ed. by Gottlieb D., Shaw P. D. 140 New York: Springer Verlag.;
    [Google Scholar]
  17. O’Connor R. J., Halvorson H. O. 1961a; The substrate specificity of l-alanine dehydrogenase.. Biochim. biophys. Acta 48:47
    [Google Scholar]
  18. O’Connor R. J., Halvorson H. O. 1961b; L-Alanine dehydrogenase: a mechanism controlling the specificity of amino acid-induced germination of Bacillus cereus spores.. J. Bact 82:706
    [Google Scholar]
  19. Powell J. f. 1957; Biochemical changes occurring during spore germination in Bacillus species.. J. appl. Bact 20:349
    [Google Scholar]
  20. Riemann H., Ordal Z. J. 1961; Germination of bacterial spores with calcium and dipicolinic acid.. Science, N. Y. 133:1703
    [Google Scholar]
  21. Rode L. J., Foster J. W. 1961; Germination of bacterial spores with alkyl primary amines.. J. Bact 81:768
    [Google Scholar]
  22. Roze U., Strominger J. L. 1966; Alanine racemase from Staphylococcus aureus: Conformation of its substrates and its inhibitor; d-cycloserine.. J. molec. Pharmacol 2:92
    [Google Scholar]
  23. Stewart B. T., Halvorson H. O. 1953; Studies on the spores of aerobic bacteria. I. The occurrence of alanine racemase.. J. Bact 65:160
    [Google Scholar]
  24. Strominger J. L., Ito E., Threnn R. H. 1960; Competitive inhibition of enzymatic reactions by Oxamycin.. J. Am. chem. Soc 82:998
    [Google Scholar]
  25. Wood W. A., Gunsalus I. C. 1951; d-Alanine formation: a racemase in Streptococcus faecalis.. J. biol. Chem 190:403
    [Google Scholar]
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