SUMMARY: The respiratory physiology of grown heterotrophically on a defined medium was examined as a function of culture age when growth was supported by glucose, acetate or ethanol as sole carbon source. The endogenous rate of oxygen consumption in general paralleled that of total protein content, usually showing a steady decline through log. and stationary phases of growth. The rate of oxygen consumption stimulated by ethanol or acetate remained fairly constant during log. phase but. decreased sharply in early stationary phase. The specific activities of representative respiratory enzymes remained essentially proportional to one another and to total oxygen consumption, but the activities of malate synthase and the malic enzyme increased greatly in stationary phase glucose-grown organisms. In general, respiratory growth was out of balance with the rate of cell division but in essential balance with the rate of biosynthesis.


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