1887

Abstract

SUMMARY: The occurrence of intergeneric transformation between staphylococci as DNA donors and Streptococcus strain challis as recipient was controlled. By various methods 126 DNA preparations were isolated from 21 staphylococcal strains resistant to different antibiotics (18 produced coagulase and DNase; three were white and did not produce coagulase or DNase). Eight of these preparations, originating from four strains of were active in intergeneric transformation. The streptomycin-resistance marker was transferred, the yield being 0.001-0.017% of that of homospecific reaction. The authors did not succeed in transferring the penicillin-, novobiocin-, erythromycin- and oxytetracyclin-resistance markers. Repeated isolations were required to produce active DNA preparations and none of the various methods yielded active preparations consistently. The staphylococcal streptomycin-resistance marker re-isolated from the transformants of strain challis was transferred to challis with the same or even higher yield than the homospecific marker.

In the staphylococcal strains from which active preparations were obtained, the GC content was 32.7-37.6% and was lower by 4.9-9% from that in strain challis (41.8%).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-53-2-187
1968-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/53/2/mic-53-2-187.html?itemId=/content/journal/micro/10.1099/00221287-53-2-187&mimeType=html&fmt=ahah

References

  1. Balassa R. 1957; Durch Desoxyribonukleinsäuren induzierte Veränderungen bei Rhizobien.. Acta microbiol. hung 4:77
    [Google Scholar]
  2. Bańkowska Z. E. 1963 Doctoral Thesis: Polska Akademia Nauk, Instytut Biochemii i Biofizyki, Warszawa.;
  3. Blobel H. 1961; Isolation and characterization of deoxyribonucleic acid from a strain of Staphylococcus aureus.. J. Bact 82:425
    [Google Scholar]
  4. Bracco R. M., Krauss M. R., Roe A. S., MacLeod C. M. 1957; Transformation reactions between pneumococcus and three strains of streptococci.. J. exp. Med 106:247
    [Google Scholar]
  5. Catlin B. W. 1964; Reciprocal genetic transformation between Neisseria catharralis and Moraxella nonliquefaciens.. J. gen. Microbiol 37:369
    [Google Scholar]
  6. Catlin B. W., Cunningham L. S. 1958; Studies of extracellular and intracellular bacterial deoxyribonucleic acids.. J. gen. microbiol 19:522
    [Google Scholar]
  7. Chen K. C., Ravin A. W. 1966; Heterospecific transformation of pneumococcus and streptococcus.. J. molec. Biol 22:109
    [Google Scholar]
  8. Dubnau D., Smith I., Morell P., Marmur J. 1965; Gene conservation in Bacillus species, I. Conserved genetic and nucleic acid base sequence homologies.. Proc. natn. Acad. Sci., U.S.A 54:491
    [Google Scholar]
  9. Hill L. R. 1966; An index to deoxyribonucleic acid base compositions of bacterial species.. J. gen. Microbiol 44:419
    [Google Scholar]
  10. Leidy G., Hahn E., Alexander H. E. 1959; Interspecific transformation in Hemophilus: a possible index of relationship between H. influenza and H. aegyptius.. Proc. Soc. exp. Med. Biol 102:86
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms.. J. molec. Biol 3:208
    [Google Scholar]
  12. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.. J. molec. Biol 5:109
    [Google Scholar]
  13. Marmur J., Falkow S., Mandel M. 1963; New approaches to bacterial taxonomy.. A. Rev. Microbiol 17:329
    [Google Scholar]
  14. Marmur J., Seaman E., Levine J. 1963; Interspecific transformation in Bacillus.. J. Bact 85:461
    [Google Scholar]
  15. Osowiecki H., Pakula R. 1962; Badania nad dezoksyrybonuleazy gronkowcową I. Pomiar aktywności dezoksyrybonukleazy gronkowcowej metodą wiskozymetryczną.. Medycyna dośw. Mikrobiol 14:173
    [Google Scholar]
  16. Pakula R. 1963; Can transformation be used as a criterion in taxonomy of bacteria?. Recent Progress in Microbiol VIII617 University of Toronto Press.:
    [Google Scholar]
  17. Pakula R., Hulanicka E., Walczak W. 1958; Transformation reactions between streptococci, pneumococci and staphylococci.. Bull. Acad. pol. Sci. Cl. II. Sér. Sci. biol 6:325
    [Google Scholar]
  18. Pakula R., Piechowska M., kowska E., Walczak W. 1962; A characteristic of DNA mediated transformation systems of two streptococcal strains.. Acta microbiol. polon 11:205
    [Google Scholar]
  19. Pakula R., Tyc M. 1956; Zastosowanie czynnika litycznego ze szczepów z rodzaju Streptomyces (aktynomycetyny) do ekstrakcji kwasu dezoksyrybonukleinowego z bakterii.. Medycyna dośw. Mikrobiol 8:523
    [Google Scholar]
  20. Schaeffer P. 1958; Interspecific reactions in bacterial transformation.. Symp. Soc. exp. Biol 12:60
    [Google Scholar]
  21. Silvestri L. G., Hill L. R. 1965; Agreement between deoxyribonucleic acid base composition and taxometric classification of Gram-positive cocci.. J. Bact. 90:136
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-53-2-187
Loading
/content/journal/micro/10.1099/00221287-53-2-187
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error