SUMMARY: Cultural conditions and supplementary substances necessary for the laboratory cultivation of some human parasitic amoebae were investigated by using a basal solution containing inorganic salts, citrate and lactate. Three supplementary components were found necessary: starch grains, animal protein (soluble or insoluble) and living bacteria. Restraint of bacterial growth by antibiotics, and of the development of an alkaline reaction (due to pellicle-forming aerobes) by carbon dioxide, improved the amoebic growth. Under these conditions, all the common parasitic amoebae examined, except , were grown and maintained for long periods in laboratory culture.


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