1887

Abstract

SUMMARY: Water-insoluble complexes of ganglioside with cerebroside fixed tetanus toxin at low concentrations (a few LD 50/ml.) of toxin. A complex containing 25% ganglioside with cerebroside was 50 times better at fixing toxin than complexes containing either 2% or 50% ganglioside. A complex containing 25% of a mixture of the gangliosides G and G was 12 times better at fixing toxin than a similar complex with gangliosides G and G. Complexes of ganglioside with sphingomyelin and lecithin fixed toxin to a slight extent, while complexes with tripalmitin and cholesterol did not fix toxin. The complex of cerebroside and ganglioside, containing 25% ganglioside, did not fix strychnine, serotonin, botulinum toxin or plasma albumin.

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/content/journal/micro/10.1099/00221287-52-3-447
1968-08-01
2024-04-19
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References

  1. Klenk E., Leupold F. 1944; Über eine vereinfachte Methode zur Darstellung von phosphor- freien Cerebrosiden und über die als Spaltprodukte auftretenden Fettsäuren. 18. Mitteilung über Cerebroside.. Hoppe-Seylers Z. physiol. Chem 281:208
    [Google Scholar]
  2. McCluer R. H., Coram E. H., Lee H. S. 1962; A silicic acid adsorption method for the determination of ganglioside sialic acid.. J. Lipid Res 3:269
    [Google Scholar]
  3. Mellanby J., Pope D. L., Ambache N. 1968; The effect of the treatment of crude tetanus toxin with ganglioside cerebroside complex on sphincter paralytic action in the rabbit’s eye.. J. gen. Microbiol 50:479
    [Google Scholar]
  4. Mellanby J., vanHeyningen W. E. 1965; The role of ganglioside in the mode of action of tetanus toxin.. Pharmacology of toxins ed. Raudonat H. Oxford, Pergamon Press.:
    [Google Scholar]
  5. Mellanby J., vanHeyningen W. E., Whittaker V. P. 1965; Fixation of tetanus toxin by subcellular fractions of brain.. J. Neurochem 12:77
    [Google Scholar]
  6. Mellanby J., Whittaker V. P. 1968; The fixation of tetanus toxin by synaptic membranes.. J. Neurochem 15:205
    [Google Scholar]
  7. Suzuki K. 1965; The pattern of mammalian brain gangliosides. II. Evaluation of the extraction procedures, post-mortem changes and the effect of formalin preservation.. J. Neurochem 12:629
    [Google Scholar]
  8. Svennerholm L. 1957; Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.. Biochim. biophys. Acta 24:604
    [Google Scholar]
  9. vanHeyningen W. E. 1959a; The fixation of tetanus toxin by nervous tissue.. J. gen. Microbiol 20:291
    [Google Scholar]
  10. vanHeyningen W. E. 1959b; Tentative identification of the tetanus toxin receptor in nervous tissue.. J. gen. Microbiol 20:310
    [Google Scholar]
  11. vanHeyningen W. E. 1963; The fixation of tetanus toxin, strychnine, serotonin and other substances by ganglioside.. J. gen. Microbiol 31:375
    [Google Scholar]
  12. vanHeyningen W. E., Miller P. A. 1961; The fixation of tetanus toxin by ganglioside.. J. gen. Microbiol 24:107
    [Google Scholar]
  13. vanHeyningen W. E., Woodman R. J. 1963; The fixation of tetanus toxin by frog brain.. J. gen. Microbiol 31:389
    [Google Scholar]
  14. Wassermann H., Takaki T. 1898; Über tetanusantitoxische Eigenschaften des normalen Zentralnervensystems.. Berl. klin. Wschr 35:5
    [Google Scholar]
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