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SUMMARY: Changes in Mycobacterium phlei due to B2h lysogeny were studied by the replica plating method. The changes could be divided into three categories: mutation to slow growth and small colony size; mutation to relative but complex nutritional requirements; and finally mutation to thiamine deficiency (thi−). A segregant clone obtained in the thi− mutant showed the characteristics of slow-growing small colony variants. Change in the bacterial host could be related to the type of integrated phage.
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