SUMMARY: Quantitative analyses of monosaccharide constituents of the cell-wall polysaccharide of the smooth form and rough mutants of Salmonella typhimurium were made by gas-liquid chromatography of glycitol acetates produced from acid hydrolysates of phenol+water extracts of cell-wall preparations. The presence, in varying amounts, of sugars characteristic of the S-specific repeating unit was detected in all rough mutants investigated. Definite conclusions about the core structure of the different rough mutants could not be drawn by using only the basis of the monosaccharide composition of the cell-wall polysaccharide. Ribose was found in the cell-wall polysaccharide of the smooth form in small amounts.
BeckmannI.,
deritzO.,
WestphalO.1964; Zur Immunchemie der somatischen Antigene von Enterobacteriaceae. IX. Serologische Typisierung von Salmonella-R-Antigenen.. Biochem. Z 339:401
BeckmannI.,
SubbaiahT. V.,
StockerB. A. D.1964; Rough mutants of Salmonella typhimurium. 2. Serological and chemical investigations.. Nature, Lond 201:1299
EdeboL.,
HolmeT.1965; Preparation of biologically active fractions of Salmonella typhimurium. 3. Extraction of immunogenic components.. Acta path, microbiol. scand 63:228
KalckarH.,
LaursenP.,
RapinA. M. C.1966; Inactivation of phage C 21 by various preparations from lipopolysaccharide of E. colik-12.. Proc. natn. Acad. Sci. U.S.A 56:1852
KauffmannF.,
gerL.,
deritzO.,
WestphalO.1961; Zur Immunchemie der O-Anti- gene von Enterobacteriaceae. VI. Vergleich der Zuckerbausteine von Polysacchariden aus Salmonella S-und R-formen.. Zentbl. Bakt. ParasitKde (Abt. I, Orig.) 182:57
KauffmannF.,
deritzO.,
StierlinH.,
WestphalO.1960; Zur Immunchemie der O-Anti- gene von Enterobacteriaceae. I. Analyse der Zuckerbausteine von Salmonella O-Antigen.. Zentbl. Bakt. ParasitKde (Abt. I, Orig.) 178:442
LüderitzO.,
BeckmannI.,
WestphalO.1964; Zur Immunchemie der somatischen Antigene von Enterobacteriaceae. X. R-spezifische Strukturen in Salmonella-O-Antigenen.. Biochem. Z 339:416
LüderitzO.,
RisseH. J.,
Schulte-HolthausenH.,
StromingerJ. L.,
SutherlandI. W.,
WestphalO.1965; Biochemical studies of the smooth-rough mutation in Salmonella minnesota
. J. Bact 89:343
LüderitzO.,
GalanosC.,
RisseH. J.,
RuschmannE.,
SchlechtS.,
SmidtG.,
HolthausenH.,
WheatR.,
WestphalO.,
SchlosshardtJ.1966; Structural relationships of Salmonella O and R antigens.. Ann. N. Y. Acad. Sci 133:349
SawardekerJ. S.,
SlonekerJ. H.,
JeanesA.1965; Quantitative determination of monosaccharides as their alditol acetates by gas liquid chromatography.. Analyt. Chem 37:1602
SutherlandI. W.,
deritzO.,
WestphalO.1965; Studies on the structure of lipopolysaccharides of Salmonella minnesota and Salmonella typhimuriumr strains.. Biochem. J 96:439