SUMMARY: strain 321V accepted the F' episome from strain 23.10S and behaved as a gene donor in crosses with several different auxotrophs of Some selected donor markers were transferred at frequencies of 10-10 per donor cell while others appeared not to be transferred. Up to 40% of recombinants were Lac+. Selected recombinants showed differing unselected marker frequencies with differing selected markers; those obtained by using double marker selection showed increased unselected marker frequencies. Some alternative explanations for the origin of recombinants (syntrophic growth, mixed clones, multiple recipient reversions) were not supported by experiment.


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