1887

Abstract

SUMMARY: Toxic and non-toxic fractions, isolated from culture supernatant fluids or from organisms of O78 K 80, were examined with the electron microscope after negative staining with phosphotungstate. Preparations of endotoxin from the supernatant fluids (‘free endotoxin’) contained large numbers of rod-like particles, considered to be the native undegraded endotoxic lipopolysaccharide-protein complex. Phenol-water extraction of either free endotoxin particles or whole organisms caused aggregation of the complex, leading to chain-like structures. Both ‘rods’ and ‘chains’ were observed in an endotoxin preparation extracted from organisms by aqueous ether. A non-toxic predominantly polysaccharidic preparation from was not visible after negative staining; but this fraction, and to a lesser extent the toxic fractions derived from culture supernatant fluids, were found to contain well-defined fragments believed to be derived from the bacterial cell wall.

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/content/journal/micro/10.1099/00221287-52-1-125
1968-06-01
2021-07-30
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References

  1. Anacker R. L., Finkelstein R. A., Haskins W. T., Landy M., Miner K. C., Ribi E., Stashak P. W. 1964; Origin and properties of naturally occurring hapten from Escherichia coli.. J. Bact 88:1705
    [Google Scholar]
  2. Bayer M. E., Anderson T. F. 1965; The surface structure of Escherichia coli.. Proc. natn. Acad. Sci. U.S.A 54:1952
    [Google Scholar]
  3. Crutchley M. J., Marsh D. G., Cameron J. 1967; Free endotoxin.. Nature, Lond 214:1052
    [Google Scholar]
  4. Crutchley M. J., Marsh S. G., Cameron J. 1968; Biological studies on free endotoxin and a non-toxic material from culture supernatant fluids of Escherichia coli O78 K80.. J. gen. Microbiol 50:413
    [Google Scholar]
  5. Knox K. W., Cullen J., Work E. 1967; An extracellular lipopolysaccharide-phospholipid- protein complex produced by Escherichia coli grown under lysine-limiting conditions.. Biochem. J 103:192
    [Google Scholar]
  6. Marsh D. G., Crutchley M. J. 1966; Bacterial lipopolysaccharides.. Br. patent application no. 48317/66
    [Google Scholar]
  7. Marsh D. G., Crutchley M. J. 1967; Purification and physico-chemical analysis of fractions from the culture supernatant of Escherichia coli O78K80: free endotoxin and a non-toxic fraction.. J. gen. Microbiol 47:405
    [Google Scholar]
  8. Ribi E., Haskins W. T., Landy M., Milner K. C. 1961; Preparation and host-reactive properties of endotoxin with low content of nitrogen and lipid.. J. exp. Med 114:647
    [Google Scholar]
  9. Ribi E., Anacker R. L., Fukushi K., Haskins W. T., Landy M., Milner K. C. 1964 Bacterial Endotoxins Ed. by Landy M., Braun W. 16 Institute of Microbiology, Rutgers University Press.:
    [Google Scholar]
  10. Rothfield L., Horne R. W. 1967; Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic and monolayer studies.. J. Bact 93:1705
    [Google Scholar]
  11. Rudbach J. A., Anacker R. L., Haskins W. T., Milner K. C., Ribi E. 1967; Physical structure of a native protoplasmic polysaccharide from Escherichia coli.. J. Immunol 98:1
    [Google Scholar]
  12. Schramm G., Westphal O., deritz O. 1952; Über bakterielle Reizstoffe. III. Mitt.: Physikalisch-chemisches Verhalten eines hochgereinigten Coli-Pyrogens.. Z. Naturf 7b:594
    [Google Scholar]
  13. Shands J. W., Graham J. A., Nath K. 1967; The morphologic structure of isolated bacterial lipopolysaccharide.. J. molec. Biol 25:15
    [Google Scholar]
  14. Taylor A., Knox K. W., Work E. 1966; Chemical and biological properties of an extracellular lipopolysaccharide from Escherichia coli grown under lysine-limiting conditions.. Biochem. J 99:53
    [Google Scholar]
  15. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia coli strain b.. J. gen. Microbiol 22:158
    [Google Scholar]
  16. Westphal O., Eichenberger E., Keiderling W. 1952; Über bakterielle Reizstoffe. I. Mitt. Reindarstellung eines Polysaccharid-Pyrogens aus Bacterium coli . Z. Naturf 7b:536
    [Google Scholar]
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