SUMMARY: The distribution of phosphorus compounds in fractions isolated from metabolically lysed yeast protoplasts by centrifugation at 2000 was examined. About 40% of the acid-soluble phosphorus compounds was associated with a particulate fraction which sedimented at 2000 and which was rich in vacuoles. This phosphorus was present largely as polyphosphates of relatively high molecular weights. Evidence is presented to support the view that this polyphosphate fraction is contained in the vacuole sap. The phosphorus of the crude vacuole fraction was labelled only slowly when the protoplasts were allowed to take up P-orthophosphate. The phosphorus entering this fraction appeared to be derived from precursors present in the 2000 supernatant fluid fraction.


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