%0 Journal Article %A Indge, K. J. %T The Isolation and Properties of the Yeast Cell Vacuole %D 1968 %J Microbiology, %V 51 %N 3 %P 441-446 %@ 1465-2080 %R https://doi.org/10.1099/00221287-51-3-441 %I Microbiology Society, %X SUMMARY: When glucose and a chelating agent such as EDTA were added to yeast protoplasts suspended in 8.5% (w/v) mannitol solution at pH 6.4 almost all the protoplasts lysed and the main vacuole was released as a discrete structure from about 75% of the protoplasts. The isolated vacuoles were themselves sensitive to osmotic shock and exhibited vital-staining reactions. They are therefore considered to be relatively undamaged. Some of the properties of the isolated vacuoles are described. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-51-3-441