1887

Abstract

SUMMARY: When glucose and a chelating agent such as EDTA were added to yeast protoplasts suspended in 8.5% (w/v) mannitol solution at pH 6.4 almost all the protoplasts lysed and the main vacuole was released as a discrete structure from about 75% of the protoplasts. The isolated vacuoles were themselves sensitive to osmotic shock and exhibited vital-staining reactions. They are therefore considered to be relatively undamaged. Some of the properties of the isolated vacuoles are described.

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/content/journal/micro/10.1099/00221287-51-3-441
1968-05-01
2021-07-26
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