1887

Abstract

SUMMARY: A technique is described for fixing and embedding dormant ascospores of , whose impermeability has made them difficult subjects for electron microscopy. The ultrastructure of dormant and germinating spores has been compared. Endoplasmic reticulum occurs relatively infrequently in dormant ascospores whereas germinating ones have large amounts. An unusual structure, consisting of concentric membranes of varying degrees of complexity, appears after activation and disappears after extension of the germ tube. This membrane complex is continuous with the endoplasmic reticulum and may be its origin during germination. Mitochondria in dormant ascospores are larger and less numerous than in germinating ones. Two new elements of the ascospore wall are described, one of which may contribute to the relative impermeability of these forms. Nuclei increase in number during germination, but the exact number of divisions is not known.

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/content/journal/micro/10.1099/00221287-51-3-403
1968-05-01
2022-05-20
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References

  1. Berliner M. D., Duff R. H. 1965; Ultrastructure of Armillaria mellae hyphae. Can. J. Bot 43:171
    [Google Scholar]
  2. Biggs D. R., Linnane A. W. 1963; The effect of oxygen on the composition and organization of the electron transport system of yeast. Biochim. biophys. Acta 78:785
    [Google Scholar]
  3. Buckley P. M., Sjaholm V. E., Sommer N. F. 1966; Electron microscopy of Botrytis cinerea conidia. J. Bad 91:2037
    [Google Scholar]
  4. Cantino E. C., Lovett J. S., Leak L. V., Lythgoe J. 1963; The single mitochondrion, fine structure, and germination of the spore of Blastocladiella emersonii. J. gen. Microbiol 31:393
    [Google Scholar]
  5. Ekundayo J. A. 1966; Further studies on germination of sporangiospores of Rhizopus arrhizus. J. gen. Microbiol 42:283
    [Google Scholar]
  6. Goddard D. R. 1935; The reversible heat activation inducing germination and increased respiration in ascospores of Neurospora tetrasperma. J. gen. Physiol 19:45
    [Google Scholar]
  7. Goddard D. R. 1939; The reversible heat activation of respiration in Neurospora. Cold Spring Harb. Symp. quant. Biol 7:362
    [Google Scholar]
  8. Hawker L. E., Abbott P. McV. 1963; An electron microscope study of maturation and germination of sporangio-spores of two species of Rhizopus. J. gen. Microbiol 32:295
    [Google Scholar]
  9. Hawker L. E., Hendy R. J. 1963; An electron-microscope study of germination of conidia of Botrytis cinerea. J. gen. Microbiol 33:43
    [Google Scholar]
  10. Holton R. W. 1960; Studies on pyruvate metabolism and cytochrome system in Neurospora tetrasperma. Plant Physiol 35:757
    [Google Scholar]
  11. Lingappa B. T., Sussman A. S. 1959; Endogenous substrates of dormant, activated and germinating ascospores of Neurospora tetrasperma. Plant Physiol 34:466
    [Google Scholar]
  12. Lingappa Y., Sussman A. S. 1959; Changes in the heat resistance of ascospores of Neurospora upon germination. Am. J. Bot 46:671
    [Google Scholar]
  13. Linnane A. W., Vitols E., Nowland P. G. 1962; Studies on the origin of yeast mitochondria. J. Cell Biol 13:345
    [Google Scholar]
  14. Lowry R. J., Sussman A. S. 1958; Wall structure of ascospores of Neurospora tetrasperma. Am. J. Bot 45:397
    [Google Scholar]
  15. Luft J. H. 1961; Improvements in epoxy embedding methods. J. biophys. biochem. Cytol 9:409
    [Google Scholar]
  16. Overman J. R., Pine L. 1963; Electron microscopy of cytoplasmic structures in facultative and anaerobic Actinomyces. J. Bact 86:656
    [Google Scholar]
  17. Prusso D. C., Wells K. 1967; Sporobolomyces roseus. I. Ultrastructure. Mycologia 59:337
    [Google Scholar]
  18. Robertson J. D. 1961; A new unit membrane organelle of Schwann cells. In Biophysics of Physiological and Pharmacological Action Ed. by Shanes A. M. no. 69 p. 63 Washington: Am. Ass. Advanc. Sci;
    [Google Scholar]
  19. Sussman A. S. 1961; The role of trehalose in the activation of dormant ascospores of Neurospora. Q. Rev. Biol 36:109
    [Google Scholar]
  20. Sussman A. S., Holton R. W., von Böventer-Heidenhain B. 1958; Physiology of the cell surface of Neurospora ascospores. Entrance of anions and non-polar compounds. Arch 29:38
    [Google Scholar]
  21. Whaley W. G., Kephart J. E., Mollenhauer H. H. 1964; The dynamics of cytoplasmic membranes during development. In Cellular Membranes in Development Ed. by Locke M. Mikrobiol p. 135 New York: Academic Press, Inc;
    [Google Scholar]
  22. Williams P. G., Ledingham G. A. 1964; Fine structure of wheat stem rust uredospores. Can. J. Bot 42:1503
    [Google Scholar]
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