1887

Abstract

SUMMARY: Cell walls were obtained from the mycelial and blastospore forms of ; these were delipidized and separated into alkali-soluble and alkali-insoluble fractions. The detailed composition of these fractions was determined in organisms grown on different media at 37° and on the same medium at different temperatures (blastospores 30°, mycelium 40°). The composition of the wall of each form was found to be constant, irrespective of growth conditions, except for some variation in the amounts of mannose and glucose in wall hydrolysates. The alkali-insoluble fraction from the mycelial form contained 3 times as much chitin as that from blastospores and only about one third as much protein. The protein from these two fractions showed marked differences in amino acid composition. Differences between the two morphological forms in the amounts of carbohydrate and protein in the alkali-soluble fractions were also found. The results are discussed in relation to other studies of cell-wall composition in the dimorphic

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-51-3-367
1968-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/51/3/mic-51-3-367.html?itemId=/content/journal/micro/10.1099/00221287-51-3-367&mimeType=html&fmt=ahah

References

  1. Anderson F. B., Millbank J. W. 1966; Protoplast formation and yeast cell wall structure. The action of the enzymes of the snail Helix pomatia. Biochem. J 99:682
    [Google Scholar]
  2. Bartnicki-Garcia S., Nickerson W. J. 1962; Isolation, composition and structure of cell walls of filamentous and yeastlike forms of Mucor rouxii. Biochim. biophys. Acta 58:102
    [Google Scholar]
  3. Bishop C. T., Blank F., Gardner P. E. 1960; The cell wall polysaccharides of Candida albicans: glucan, mannan and chitin. Can. J. Chem 38:869
    [Google Scholar]
  4. Dubos R. J., Hirsch J. G. 1965 In Bacterial and Mycotic Infections of Man p. 836 Philadelphia: Lippincott;
    [Google Scholar]
  5. Eddy A. A. 1958; The structure of the yeast cell wall. II. Degradative studies with enzymes. Proc. R. Soc B 149:425
    [Google Scholar]
  6. Fiske C. H., SubbaRow Y. 1925; The colorimetric determination of phosphorus. J. biol. Chem 66:375
    [Google Scholar]
  7. Hill D. W., Gebhardt L. P. 1956; Morphological transformation of Candida albicans in tissues of mice. Proc. Soc. exp. biol. Med 92:640
    [Google Scholar]
  8. Houwink A. L., Kreger D. R., Roelofsen P. A. 1951; Composition and structure of yeast cell walls. Nature, Lond 168:693
    [Google Scholar]
  9. Johnston I. R. 1965; The composition of the cell wall of Aspergillus niger. Biochem. J 96:651
    [Google Scholar]
  10. Kessler G., Nickerson W. J. 1959; Glucomanann–protein complexes from cell walls of yeasts. J. biol. Chem 234:2281
    [Google Scholar]
  11. Kobayashi G. S., Guiliacci P. L. 1967; Cell wall studies of Histoplasma capsulatum. Sabouraudia 5:180
    [Google Scholar]
  12. Korn E. D., Northcote D. H. 1960; Physical and chemical properties of polysaccharide sand glycoproteins of the yeast cell wall. Biochem. J 75:12
    [Google Scholar]
  13. Mariat F. 1964; Saprophytic and parasitic morphology of pathogenic fungi. Symp. Soc. gen. Microbiol 14:85
    [Google Scholar]
  14. Marks V. 1959; An improved glucose oxidase method for determining blood, C.S.F. and urine glucose levels. Clinica chim. Acta 4:395
    [Google Scholar]
  15. Mejbaum W. 1939; Ǖber die Bestimmung kleiner Pentosemengen, insbesondere in Derivaten der Adenylsäure. Z. physiol. Chem 258:117
    [Google Scholar]
  16. Moore S., Stein W. H. 1954; A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. biol. Chem 211:907
    [Google Scholar]
  17. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. J. biol. Chem 153:375
    [Google Scholar]
  18. Nickerson W. J. 1954; An enzymatic locus participating in cellular division of a yeast. J. gen. Physiol 37:483
    [Google Scholar]
  19. Nickerson W. J. 1963; Symposium on biochemical basis of morphogenesis in fungi. IV. Molecular bases of form in yeasts. Bact. Rev 27:305
    [Google Scholar]
  20. Nickerson W. J., Falcone G. 1956; Identification of protein disulphide reductase as a cellular division enzyme in yeasts. Science, N. Y 124:722
    [Google Scholar]
  21. Nickerson W. J., Mankowski Z. 1953; A polysaccharide medium of known composition favouring chlamydospore formation in Candida albicans. J. inf. Dis 92:20
    [Google Scholar]
  22. Phaff H. J. 1963; Cell wall of yeasts. Ann. Rev. Microbiol 17:15
    [Google Scholar]
  23. Roelofsen P. A., Hoette I. 1951; Chitin in the cell wall of yeasts. Antonie van Leeuwenhoek 17:297
    [Google Scholar]
  24. Rogers K. B. 1957; An aid to the differentiation between cases of moniliasis and monilic carriers. J. clin. Pathol 10:406
    [Google Scholar]
  25. Roth F. J., Goldstein M. I. 1961; Inhibition of growth of pathogenic yeasts by human serum. J. invest. Derm 36:383
    [Google Scholar]
  26. Seelig M. S. 1966; Mechanisms by which antibiotics increase the incidence and severity of candidiasis and alter the immunological defences. Bact. Rev 30:442
    [Google Scholar]
  27. Smith I. 1960 In Chromatographic and Electrophoretic Techniques Ed. by Smith Ivor. vol. 1 p. 85 London: Heinemann;
    [Google Scholar]
  28. Taschdjian C. L., Kozinn P. J. 1957; Laboratory and clinical studies on candidiasis in the newborn infant. J. Pediatr 50:426
    [Google Scholar]
  29. Tracey M. V. 1955 In Modern Methods of Plant Analysis Ed. by Paech K., Tracey M. V. vol. 11 p. 264 Berlin: Springer-Verlag;
    [Google Scholar]
  30. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature, Lond 166:444
    [Google Scholar]
  31. Van Uden N. 1960; The occurrence of Candida and other yeasts in the intestinal tracts of animals. Ann. N.Y. Acad. Sci 89:59
    [Google Scholar]
  32. Widra A. 1964; Phosphate directed Y-M variation in Candida albicans. Mycopath. Mycol. appl 23:197
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-51-3-367
Loading
/content/journal/micro/10.1099/00221287-51-3-367
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error