Use of Inhibitors for Selective Isolation and Enumeration of Cytophagas from Natural Substrates Free

Abstract

Several antibacterial agents were tested against a range of cytophagas and other bacteria representing types commonly found in soil and marshy places. From these tests two antibiotics (penicillin 15 units/ml. and chloramphenicol 5.0 μg./ml.) and two bile salts (sodium taurocholate 8.0 mg./ml. and sodium cholate 6.0 mg./ml.) were selected for incorporation into alkaline enrichment media to achieve selective growth of cytophagas. This mixture of antibiotics and bile salts was tested with a number of soils and its efficiency compared with several other methods for selective development of cytophagas; bile salts inhibited two common species. The most suitable mixture for the selection of cytophagas was enrichment medium with penicillin and chloramphenicol.

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/content/journal/micro/10.1099/00221287-51-1-43
1968-04-01
2024-03-28
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References

  1. Bachmann B. J. 1955; Studies on Cytophaga fermentans, n.sp., a facultatively anaerobic lower myxobacterium. J. gen. Microbiol 13:541
    [Google Scholar]
  2. Brockman E. R., Boyd W. L. 1963; Myxobacteria from soils of the Alaskan and Canadian Arctic. J. Bact 86:605
    [Google Scholar]
  3. Fuller W. H., Norman A. G. 1942; A cellulose-dextrin medium for identifying cellulose organisms in soil. Proc. Soil Sci. Soc. Am 7:243
    [Google Scholar]
  4. Fuller W. H., Norman A. G. 1943; Characteristics of some soil cytophagas. J. Bact 45:565
    [Google Scholar]
  5. Stanier R. Y. 1942; The Cytophaga group: a contribution to the biology of myxobacteria. Bact. Rev 6:143
    [Google Scholar]
  6. Stanier R. Y. 1947; Studies on nonfruiting myxobacteria. I. Cytophaga johnsonae, n.sp., a chitin- decomposing myxobacterium. J. Bact 53:297
    [Google Scholar]
  7. Veldkamp H. 1961; A study of two marine agar-decomposing, facultatively anaerobic myxobacteria. J. gen. Microbiol 26:331
    [Google Scholar]
  8. Warke G. M., Dhala S. A. 1966; Effect of heat and antimicrobial agents on Cytophaga sp. isolated from various substrates in Bombay. Indian J. Microbiol 6:5
    [Google Scholar]
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