Several antibacterial agents were tested against a range of cytophagas and other bacteria representing types commonly found in soil and marshy places. From these tests two antibiotics (penicillin 15 units/ml. and chloramphenicol 5.0 μg./ml.) and two bile salts (sodium taurocholate 8.0 mg./ml. and sodium cholate 6.0 mg./ml.) were selected for incorporation into alkaline enrichment media to achieve selective growth of cytophagas. This mixture of antibiotics and bile salts was tested with a number of soils and its efficiency compared with several other methods for selective development of cytophagas; bile salts inhibited two common species. The most suitable mixture for the selection of cytophagas was enrichment medium with penicillin and chloramphenicol.


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