1887

Abstract

SUMMARY: The structure of surface layers of the bacterial form and different types of the L-form of was studied by electron microscopy of thin-sectioned organisms. Morphological data confirm the distinction between the cell wall-less protoplast-L-form and the wall-containing spheroplast-L-form. Organisms of the protoplast-L-form have only one surface-integument, presumably the cytoplasmic membrane. These forms never revert to the bacterial form. The spheroplast-L-form comprises reversible forms (unstable-sphero-plast-L-form) as well as non-reverting strains (stable spheroplast-L-form). In both spheroplast-L-forms two surface integuments are always present: a cytoplasmic membrane and a superposed cell wall. In sections of isolated cell walls of normal Proteus bacteria and in wall material spontaneously dissociating from damaged cell walls of spheroplast-L-forms the triple-layered ‘unit membrane’ is a prominent feature. Thin sections of isolated cell wall lipopolysaccharide identify the unit membrane as a specific structure of this polymer. The thickness of the murein (syn. mucopeptide, mucopolymer) in isolated murein layers (‘murein sacculi’, Weidel & Pelzer, 1964) from cell walls of normal Proteus bacteria was found to be approximately 20–25 å.

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1968-04-01
2024-04-25
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References

  1. Cota-Robles E. H. 1963; Electron microscopy of plasmolysis in Escherichia coli. J. Bad 85:549
    [Google Scholar]
  2. Davis B. D. 1966; Proposal made at ‘Brook Lodge Conference on Microbial Protoplasts, Sphero- plasts and L-forms’. Augusta, Mich.; U.S.A:
    [Google Scholar]
  3. Fishman D. A., Weinbaum G. 1967; Hexagonal pattern in cell walls of Escherichia coli b. Science, N. Y 155:472
    [Google Scholar]
  4. Frank H., Dekegel D. 1965; Zur Interpretation von Zellwand-Strukturen in Dunnschnitten Gram-negativer Bakterien. Zentbl. Bakt Abt., I 198:81
    [Google Scholar]
  5. Frank H., Dekegel D. 1967; Electron microscopical studies on the localization of the different components of cell walls of Gram-negative bacteria. Folia microbiol., Praha 12:221
    [Google Scholar]
  6. Hofschneider P. H. 1960; Zur Wandstruktur von Escherichia coli b Sphaeroplasten. Proc. Eur. Reg. Conf. Electron Microscopy, Delft. vol. 2:1028
    [Google Scholar]
  7. Hofschneider P. H., Lorek H. 1962; Studies on the residual cell wall structures of E. coli and B. megaterium spheroplasts and of L-forms of Proteus mirabilis. Proc. 5th int. Congr. Electron Microscopy, PhiladelphiaRR9
    [Google Scholar]
  8. Kandler O., Kandler G. 1956; Trennung und Charakterisierung verschiedener L-Phasen-Typen von Proteus vulgaris. Z. Naturf 11b:252
    [Google Scholar]
  9. Kandler O., Zehender C. 1957; über das Vorkommen von α, ε-Diaminopimelinsaure bei verschiedenen L-Phasentypen von Proteus vulgaris und bei den pleuropneumonieahnlichen Organismen. Z. Naturf 12b:725
    [Google Scholar]
  10. Kandler O., Hund A., Zehender C. 1958; Cell wall composition in bacterial and L-forms of Proteus vulgaris. Nature, Lond 181:572
    [Google Scholar]
  11. Kellenberger E. 1952; Die Einflüsse verschiedener Prӓparationsmethoden auf E. coli b. Z. wiss. Mikrosk 60:408
    [Google Scholar]
  12. Kellenberger E., Ryter A. 1958; Cell wall and cytoplasmic membrane of Escherichia coli. J. biophys. biochem. Cytol 4:323
    [Google Scholar]
  13. Klieneberger-Nobel E. 1960; L-forms of Bacteria. In The Bacteria Ed. by Gunsalus I. C., Stanier R. Y. Vol. 1 p. 363 New York and London: Academic Press;
    [Google Scholar]
  14. Knox K. W., Vesk M., Work E. 1966; Relation between excreted lipopolysaccharide complexes and surface structures of a lysine limited culture of Escherichia coli. J. Bact 92:1206
    [Google Scholar]
  15. Lieve L. 1965a; Release of lipopolysaccharide by EDTA treatment of E. coli. Biochem. biophys. Res. Comm 21:290
    [Google Scholar]
  16. Lieve L. 1965b; Actinomycin sensitivity in Escherichia coli produced by EDTA. Biochem. biophys. Res. Comm 18:13
    [Google Scholar]
  17. Martin H. H. 1963; Ober den Aufbau der Zellwand bei Bakterien und L-Formen von Proteus mirabilis. Habilitationsschrift: Technische Hochschule Munchen;
    [Google Scholar]
  18. Martin H. H. 1964; Composition of the mucopolymer in cell walls of the unstable and stable L-form of Proteus mirabilis. J. gen. Microbiol 36:441
    [Google Scholar]
  19. Martin H. H. 1967; Murein structure in cell walls of normal bacteria and L-forms of Proteus mirabilis and the site of action of penicillin. Folia microbiol., Praha 12:234
    [Google Scholar]
  20. Martin H. H., Frank H. 1962; Quantitative Bausteinanalyse der Stiitzmembran in der Zellwand von Escherichia coli b. Z. Naturf 17b:190
    [Google Scholar]
  21. Murray R. G. E., Steed P., Elson H. E. 1965; The location of the mucopetide in sections of the cell wall of Escherichia coli and other Gram-negative bacteria. Can. J. Microbiol 11:547
    [Google Scholar]
  22. de Petris S. 1965; Ultrastructure of the cell wall of Escherichia coli. J. Ultrastructure Res 12:247
    [Google Scholar]
  23. Polsinelli M., Ciferri O., Cassani G., Albertini A. 1964; Mechanism of resistance to actinomycin in Bacillus subtilis. J. Bact 88:1567
    [Google Scholar]
  24. Robertson I. D. 1959; The ultrastructure of cell membranes. Symp. Biochem. Soc 16:3
    [Google Scholar]
  25. Ryter A., Kellenberger E. 1958; Les nucléoides des bact6ries en croissance active. Z. Naturf 13b:597
    [Google Scholar]
  26. Schramm G., Westphal O., Lüderitz O. 1952; über bakterielle Reizstoffe. III. Physikalisch- chemisches Verhalten eines hochgereinigten Coli-Pyrogens. Z. Naturf 7b:594
    [Google Scholar]
  27. Taubeneck U. 1961; Die Phagenresistenz der stabilenL-Formvon Proteus mirabilis. Z. Naturf 16b:849
    [Google Scholar]
  28. Taubeneck U., Bohme H., Schumann G. 1958; Untersuchungen iiber die L-Phase von Proteus mirabilis mit Hilfe von Bacteriophagen. Biol. Zbl 77:663
    [Google Scholar]
  29. Tulasne R., Minck R., Kirn A. 1962; étude comparative, au microscope dlectronique, d’un Proteus et des formes L des types A et B corréspondantes. Annls Inst. Pasteur, Paris 102:292
    [Google Scholar]
  30. Weibull C. 1965; Structure of bacterial L-forms and their parent bacteria. J. Bact 90:1467
    [Google Scholar]
  31. Weidel W. 1958; Bacterial viruses. A. Rev. Microbiol 12:27
    [Google Scholar]
  32. Weidel W., Kellenberger E. 1955; The E. coli B-receptor for the phage T5. II. Electron microscopic studies. Biochim. biophys. Acta 17:1
    [Google Scholar]
  33. Weidel W., Pelzer H. 1964; Bag-shaped macromolecules—a new outlook on bacterial cell walls. Adv. Enzymol 26:193
    [Google Scholar]
  34. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia coli strain b.. J. gen. Microbiol 22:158
    [Google Scholar]
  35. Weidel W., Koch G., Bobosch K. 1954; über die Rezeptorsubstanz fur den Phagen T5. I. Extraktion und Reindarstellung aus E. coli b., Z. Naturf 9b:573
    [Google Scholar]
  36. Weidel W., Koch G., Lohss F. 1954; Ober die Zellmembran von E. coli b. II. Der Rezeptor- komplex für die Bakteriophagen T3, T4 and T7. Z. Naturf 9b:398
    [Google Scholar]
  37. Zickler F. 1967; Phagenrezeptorsynthese durch die stabile L-Form von Proteus mirabilis VI. Z. allg. Mikrobiol 7:283
    [Google Scholar]
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