1887

Abstract

SUMMARY: Excystment agents (extract of , living and glutamic acid) failed to cause excystment of cysts in the presence of either emetine or compounds I and II (structurally based on emetine). A very high percentage of the cysts excysted in the presence of the excystment agents after the removal of emetine, showing that the treated cysts were viable. Ninhydrin reaction, using a fixed amount of glutamic acid and increasing concentrations of emetine, showed progressive inhibition of colour development. This suggests the possible binding of excystment factor to emetine, thus preventing excystment. Excystment inducing property of the excystment agents could not be prevented in the presence of carbarsone. When the cysts were treated with sodium lauryl sulphate and then with emetine or with sodium lauryl sulphate and emetine together, there was hardly any excystment. Sodium lauryl sulphate rendered the cyst wall permeable to emetine and the latter killed the cysts.

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/content/journal/micro/10.1099/00221287-51-1-17
1968-04-01
2022-01-23
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