1887

Abstract

SUMMARY: The formation of chlorophyll in non-proliferating etiolated cells of var. is inhibited by some antimetabolite analogues of vitamins. The cell size and cell mass of light-grown euglenas is considerably increased in vitamin B 12 deficiency. The inhibitory effect of 2,6-diaminopurine on chlorophyll formation in non-proliferating euglenas was not annulled by vitamin B 12; 6-mercaptopurine, sulphanilamide and benzimidazole were without effect. Isoniazide inhibition is not reversed by niacin: surprisingly, the vitamin itself is markedly inhibitory. The inhibitory effect of niacin, however, is prevented by pyridine-3-sulphonate. Niacin and its analogues were more inhibitory to growth in the dark than in the light. The inhibition of growth by niacin in the light is annulled appreciably by either glucose or pyruvate, or an overwhelming concentration of vitamin B 12. Aminopterin, desoxypyridoxine and 2-chloro--aminobenzoic acid do not have any effect on chlorophyll synthesis in non-proliferating euglenas; the last mentioned inhibits the growth of the alga more in the dark than in the light. Thiamine deficiency inhibits growth; such sub-optimally grown euglenas also synthesize less chlorophyll per cell on subsequent illumination under non-proliferation conditions.

The negative growth response of light-grown var. to niacin suggests a microbiological method of estimating this vitamin in biological materials and pharmaceutical preparations up to concentrations of 70 μg./ml. in the growth medium. The 50% inhibition level of niacin in the light is 46 μg./ml.

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1968-04-01
2024-03-29
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References

  1. Beck W. S., Hurlock B., Levin M. 1960; Unbalanced growth in Lactobacillus leichmanii cultivated in excess and limiting vitamin B 12 and deoxyriboside. Fedn. Proc 19:417
    [Google Scholar]
  2. Brawerman G., Konigsberg N. 1960; On the formation of theTPN requiring glyceraldehyde-3-phosphate dehydrogenase during the production of chloroplasts in Euglena gracilis. Biochim. biophys. Acta 43:374
    [Google Scholar]
  3. Buetow D. E. 1962; Differential effects of temperature on the growth of Euglena gracilis. Expl. Cell Res 27:137
    [Google Scholar]
  4. Coelho J. 1966; Studies in vitamin B 12 metabolism. Thesis, Ph.D. (Tech.) University of Bombay;
    [Google Scholar]
  5. Coelho J., Dubash P. J., Rege D. V. 1964; Studies on the Euglena gracilis assay method for vitamin B 12. Bull. Natn. Inst. Sci. India no. 25 p. 201
    [Google Scholar]
  6. Cramer M., Myers J. 1952; Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol 17:384
    [Google Scholar]
  7. Dubash P. J., Rege D. V. 1967a; Chlorophyll formation in Euglena gracilis var. bacillaris. Interference by analogues of purines, pyrimidines and amino acids. J. gen. Microbiol 48:283
    [Google Scholar]
  8. Dubash P. J., Rege D. V. 1967b; Permanent bleaching of Euglena by Mg2+ starvation. Biochim. biophys. Acta 136:185
    [Google Scholar]
  9. Dubnoff J. W., Barton E. 1956; Activation of protein sulphydryl groups by vitamin B 12. Archs. Biochem. Biophys 62:86
    [Google Scholar]
  10. Epstein S. S., Timmis G. M. 1963; Simple antimetabolites of vitamin B 12. J. Protozool 10:63
    [Google Scholar]
  11. Epstein S. S., Weiss J. B. 1960a; Measuring the size of isolated cells. Nature, Lond 187:461
    [Google Scholar]
  12. Epstein S. S., Weiss J. B. 1960b; The extraction of pigments from Euglena gracilis. Biochem. J 75:247
    [Google Scholar]
  13. Epstein S. S., Weiss J. B., Causeley D., Bush P. 1962; Influence of vitamin B 12 on the size and growth of Euglena gracilis. J. Protozool 9:336
    [Google Scholar]
  14. Ford J. E., Goulden J. D. S. 1959; The influence of vitamin B 12 on the growth rate and cell composition of the flagellate Ochromonas malhamensis. J. gen. Microbiol 20:267
    [Google Scholar]
  15. Frank O., Baker H., Ziffer H., Aaronson S., Hutner S. H., Leevy C. M. 1963; Metabolic deficiencies in protozoa induced by thalidomide. Science, N.Y 139:110
    [Google Scholar]
  16. Fuller R. C., Gibbs M. 1959; Intracellular and phylogenetic distribution of ribulose 1:5-diphosphate carboxylase and d-glyceraldehyde 3-phosphate dehydrogenase. PL Physiol 34:324
    [Google Scholar]
  17. Hutner S. H. 1961; Plant animals as experimental tools for growth studies. Bull. Torrey Bot. Club 88:339
    [Google Scholar]
  18. Hutner S. H., Provasoli L. 1964; Nutrition of algae. A. Rev. Pl. Physiol 15:37
    [Google Scholar]
  19. Hutner S. H., Bach M. K., Ross G. I. M. 1956; A sugar-containing basal medium for vitamin B 12-assay with Euglena: application to body fluids. J Protozool 3:101
    [Google Scholar]
  20. Hutner S. H., Provasoli L., Schatz A., Haskins C. 1950; Some approaches to the study of the role of metals in the metabolism of micro-organisms. Proc. Am. Phil. Soc 94:152
    [Google Scholar]
  21. Rueckert R. R., Mueller G. C. 1960; Studies on unbalanced growth in tissue culture. I. Induction and consequence of thymidine deficiency. Cancer Res 20:1584
    [Google Scholar]
  22. Schopfer W. H., Keller V. 1951; Action of vitamin Ks and nicotinic acid on the green and colourless forms of Euglena gracilis. Bull. soc. chim biol 33:1253
    [Google Scholar]
  23. Venkataraman S., Netrawali M. S., Sreenivasan A. 1965; The role of vitamin B 12 in the metabolism of Euglena gracilis var. bacillaris. Biochem. J 96:552
    [Google Scholar]
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