The Recombination of Bromouracil-containing Deoxyribonucleic Acid in Transduction Free

Abstract

SUMMARY: tolerated the replacement of about 70% of the thymine in the DNA by 5-bromouracil (BU). In phage PLT-22 transduction, the presence of BU in the donor DNA had no effect on the linkage relationship of two contransduced markers and did not conclusively alter the ratio of complete transductants to abortive transductants. The yield of transductants per plaque-forming unit was more than doubled by the treatment of the donor bacteria and phage with BU. However, more than half of the BU-treated phage particles, detected by electron microscopy, did not form plaques; when the multiplicity of infection was calculated from the titre of physical particles, the frequency of transductants was normal. It is concluded that recombination was not affected by the substantial incorporation of BU into the donor DNA. Treatment of the recipient bacteria with BU slightly modified recombination by decreasing the frequency of transductants by about 40% and the linkage of the cotransduced markers by about 5%. Because comparable results were not obtained when the donor DNA contained BU, it is suggested that these effects resulted indirectly from the treatment of the recipient bacteria.

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1968-04-01
2024-03-28
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References

  1. Bertani G. 1958; Lysogeny. Adv. Virus Res 5:151
    [Google Scholar]
  2. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochim. biophys. Acta 34:103
    [Google Scholar]
  3. Dunn D. B., Smith J. D. 1954; Incorporation of halogenated pyrimidines into the deoxyribonucleic acids of Bacterium coli and its bacteriophages. Nature, Lond 174:305
    [Google Scholar]
  4. Dunn D. B., Smith J. D. 1957; Effects of 5-halogenated uracils on the growth of Escherichia coli and their incorporation into deoxyribonucleic acids. Biochem. J 67:494
    [Google Scholar]
  5. Ephrati-Elizur E., Zamenhof S. 1959; Incorporation of 5-bromouracil into transforming principle of Bacillus subtilis and its biological effects. Nature, Lond 184:472
    [Google Scholar]
  6. Folsome C. E. 1960; Effects of 5-bromodesoxyuridine upon gene recombination in Escherichia coli k-12. Genetics 45:1111
    [Google Scholar]
  7. Gimlin D. M., Hardman S. D., Kelley B. N., Butler G. C., Leach F. R. 1966; Effect of bromouracil-containing deoxyribonucleic acid on Bacillus subtilis. J. Bact 92:366
    [Google Scholar]
  8. Greer S. 1960; Studies on ultraviolet irradiation of Escherichia coli containing 5-bromouracil in its DNA. J. gen. Microbiol 22:618
    [Google Scholar]
  9. Ikeda H., Tomizawa J. 1965; Transducing fragments in generalized transduction by phage Pi. I. Molecular origin of the fragments. J. molec. Biol 14:85
    [Google Scholar]
  10. Jacob F., Brenner S., Cuzin F. 1963; On the regulation of DNA replication in bacteria. Cold Spring Harb. Symp. quant. Biol 28:329
    [Google Scholar]
  11. Loring H. S. 1955; Hydrolysis of nucleic acids and procedures for the direct estimation of purine and pyrimidine fractions by absorption spectrophotometry. In The Nucleic Acids Ed. by Chargaff E., Davidson J. N. vol. 1 p. 191 New York and London: Academic Press;
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. molec. Biol 3:208
    [Google Scholar]
  13. Ozeki H. 1956; Abortive transduction in purine-requiring mutants of Salmonella typhimurium. Genetic studies with bacteria. Pubis. Carnegie Instn no. 612 p. 97
    [Google Scholar]
  14. Shchipkov V. P., Samoylenko L. I. 1966; The effect of 5-bromouracil upon gene recombination in Escherichia coli depending on the amount of thymine it replaces in the DNA of donor cells. Genetika 12:99 (Orig. in Russian.)
    [Google Scholar]
  15. Smith-Keary P. F. 1960; A suppressor of leucineless in Salmonella typhimurium. Heredity 14:61
    [Google Scholar]
  16. Szybalski W., Opara-Kubinska Z., LorkieWicz L., Ephrati-Elizur E., Zamenhof S. 1960; Transforming activity of deoxyribonucleic acid labelled with 5-bromouracil. Nature, Lond 188:743
    [Google Scholar]
  17. Witkin E. M. 1956; Time, temperature and protein synthesis: a study of ultraviolet-induced mutation in bacteria. Cold Spring Harb. Symp. quant. Biol 21:123
    [Google Scholar]
  18. Wyatt G. R. 1951; The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem. J 48:584
    [Google Scholar]
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