1887

Abstract

SUMMARY

Factors concerned in demonstrating haemadsorption and haemagglutination and their occurrence among different mycoplasmas were investigated. Haemadsorption occurred best to colonies which had recently developed on agar at pH 6·5. Mycoplasmas isolated from various bird and animal sources, e.g. , , and , haemadsorbed with erythrocytes from a wide range of species. However, not all strains within a serotype haemadsorbed. Thus, the ‘Negroni’ strain of did not. Haemadsorption could be inhibited by crowding of colonies on agar and by the addition of specific antiserum to the colonies. Generally, antiserum titres obtained by haemadsorption inhibition were low in comparison with those obtained by metabolic inhibition, and haemadsorption inhibition was not useful as a routine serological technique.

The development of the haemagglutinin of in liquid medium was studied in detail; a change in the pH value of the medium could be used as an index of its development and it was intimately associated with the organism. The centrifuged deposits of other mycoplasmas, from birds, cattle, goats, man, rodents and pigs, and which were grown in liquid medium also haemagglutinated, but generally to low titre. Haemagglutination occurred best in 𝖴-shaped cups at 37° and at pH 6·5–7·0 and could be inhibited by specific antiserum. There was lack of correlation between haemadsorption and haemagglutination; both these phenomena were exhibited by some mycoplasmas, others haemadsorbed only, and still others haemagglutinated only.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-50-3-465
1968-03-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/50/3/mic-50-3-465.html?itemId=/content/journal/micro/10.1099/00221287-50-3-465&mimeType=html&fmt=ahah

References

  1. Berg R. B., Frothingham T. E. 1961; Haemadsorption in monkey kidney cell cultures of Myco-plasma (PPLO) recovered from rats. Proc. Soc. exp. Biol. Med 108616
    [Google Scholar]
  2. Butler M., Leach R. H. 1964; A mycoplasma which induces acidity and cytopathic effect in tissue culture. J. gen. Microbiol 34:285
    [Google Scholar]
  3. Chanock R. M., Hayflick L., Barile M. F. 1962; Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc. natn. Acad. SciU.S.A 4841
    [Google Scholar]
  4. Chu H. P., Horne R. W. 1967; Electron microscopy of M. gallisepticum and M. mycoides using the negative staining technique and their comparison with myxovirus. Ann. N. Y. Acad. Sci 143:190
    [Google Scholar]
  5. Clarke D. H., Casals J. 1958; Techniques for haemagglutination and haemagglutination inhibition with arthropod-borne viruses. Am. J. trop. Med. Hyg 7:561
    [Google Scholar]
  6. Clyde W. A., Jun. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immun 92:958
    [Google Scholar]
  7. Del Giudice R. A., Pavia R. 1964; Hemadsorption by Mycoplasma pneumoniae and its inhibition with sera from patients with atypical pneumonia. Bact. Proc71
    [Google Scholar]
  8. Dierks R. E., Newman J. A., Pomeroy B. S. 1967; Characterization of avian mycoplasma. Ann. N.Y. Acad. Sci 143:170
    [Google Scholar]
  9. Edward D. G. ff., Freundt E. A. 1965; A note on the taxonomic status of strains like ‘Campo’ hitherto classified as Mycoplasma hominis type 2. J. gen. Microbiol 41:263
    [Google Scholar]
  10. Fallon R. J., Grist N. R., Inman D. R., Lemcke R. M., Negroni G., Woods D. A. 1965; Further studies of agents isolated from tissue cultures incoculated with human leukaemic bone marrow. Br. med. J ii:388
    [Google Scholar]
  11. Feldman H. A., Suhs R. H. 1966; Serologic epidemiologic studies with M. pneumoniae. I. Demonstration of an haemagglutinin and its inhibition by antibody. Am. J. Epidemiol 83:345
    [Google Scholar]
  12. Gesner B., Thomas L. 1965; Sialic acid binding sites: role in hemagglutination by Mycoplasma gallisepticum . Science, N. Y 151:590
    [Google Scholar]
  13. Gourlay R. N., Shifrine M. 1966; Antigenic cross-reactions between the galactan from Mycoplasma mycoides and polysaccharides from other sources. J. comp. Path. Ther 76:417
    [Google Scholar]
  14. Hayflick L. 1965; Tissue cultures and mycoplasmas. Tex. Rep. Biol. Med Suppl. 1 23:288
    [Google Scholar]
  15. John T. J., Stahl M., Fulginiti V. A. 1966; Cumulative hemagglutination by Mycoplasma pneumoniae and other agglutinins. J. Bact 92:1002
    [Google Scholar]
  16. Jungherr E. L., Luginbuhl R. E., Jacobs R. E. 1953; Pathology and serology of air sac infection. Proc. Am. vet. Med. Ass. 90th ann. meet303
    [Google Scholar]
  17. Leach R. H., Butler M. 1966; Comparison of mycoplasmas associated with human tumours, leukemia, and tissue cultures. J. Bact 91:934
    [Google Scholar]
  18. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. 1966b; A color test for the measurement of antibody to the non-acid-forming mycoplasma species. Am. J. Epidemiol 84:51
    [Google Scholar]
  19. Purcell R. H., Somerson N. L., Fox H., Wong D. C, Turner H. C, Chanock R. M. 1966a; Identification of acid-inducing agent and related mycoplasmas as M. hyorhinis . J. natn. Cancer Inst 37:251
    [Google Scholar]
  20. Roberts D. H. 1964; Serotypes of avian mycoplasma. J. comp. Path. Ther 74:447
    [Google Scholar]
  21. Roberts D. H. 1967; Neuraminidase-like enzyme present in Mycoplasma gallisepticum . Nature, Lond 213:87
    [Google Scholar]
  22. Somerson N. L., Walls B. E., Chanock R. M. 1965; Hemolysin of Mycoplasma pneumoniae tentative identification as a peroxide. Science, N. Y 150:226
    [Google Scholar]
  23. Somerson N. L., Purcell R. H., Taylor-Robinson D., Chanock R. M. 1965; Hemolysin of Mycoplasma pneumoniae . J. Bact 89:813
    [Google Scholar]
  24. Taylor-Robinson D., Manchee R. J. 1967a; Adherence of mycoplasmas to glass and plastic. J. Bact 94:1781
    [Google Scholar]
  25. Taylor-Robinson D., Manchee R. J. 1967b; Spermadsorption and spermagglutination by mycoplasmas. Nature Lond 215:484
    [Google Scholar]
  26. Taylor-Robinson D., Purcell R. H., Wong D. C, Chanock R. M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg., Camb 64:91
    [Google Scholar]
  27. Van Herck W., Eaton M. D. 1945; An unidentified pleuro-pneumonia-like organism isolated during passages in chick embryos. J. Bad 50:47
    [Google Scholar]
  28. Zucker-Franklin D., Davidson M., Thomas L. 1966a; The interaction of mycoplasmas with mammalian cells. I. Hela cells, neutrophils, and eosinophils. J. exp. Med 124:521
    [Google Scholar]
  29. Zucker-Franklin D., Davidson M., Thomas L. 1966b; The interaction of mycoplasmas with mammalian cells. II. Monocytes and lymphocytes. J. exp. Med 124:533
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-50-3-465
Loading
/content/journal/micro/10.1099/00221287-50-3-465
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error