1887

Abstract

SUMMARY

Chloramphenicol was found to inhibit the growth of , showing a maximum dose-response at a concentration of 100 g./ml. Mycelium grown in the presence of chloramphenicol 100 g./ml., was devoid of the mitochondrial cytochromes and but contained increased cytoplasmic cytochrome Mitochondria in thin sections of hyphae grown in the presence of chloramphenicol resembled mitochondria in normal hyphae. In negatively stained preparations of isolated normal mitochondria stalked particles were observed on the cristae. These particles could not be demonstrated in mitochondria from hyphae grown in the presence of chloramphenicol 100g./ml.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-50-3-391
1968-03-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/50/3/mic-50-3-391.html?itemId=/content/journal/micro/10.1099/00221287-50-3-391&mimeType=html&fmt=ahah

References

  1. Bretthauer R. K., Marcus L., Chaloupka J., Halvorson H. O., Bock R. M. 1963; Amino acid incorporation into protein by cell-free extracts of yeast. Biochemistry, N. Y 2:1079
    [Google Scholar]
  2. Ehrenstein G. von, Lipmann F. 1961; Experiments on hemoglobin biosynthesis. Proc. natn. Acad. SciU.S.A 47941
    [Google Scholar]
  3. Fernández-Morán H., Oda T., Blair P. V., Green D. E. 1964; A macromolecular repeating unit of mitochondrial structure and function. Correlated electron microscopic and biochemical studies of isolated mitochondria and submitochondrial particles of beef heart muscle. J. Cell Biol 22:63
    [Google Scholar]
  4. Green D. E., Perdue J. F. 1966; Correlation of mitochondrial structure and function. Ann. N. Y. Acad. Sci 137:667
    [Google Scholar]
  5. Hackenbrock C. R. 1966; Ultrastructural bases for metabolically linked mechanical activity in mitochondria. I. Reversible ultrastructural changes with change in metabolic steady state in isolated liver mitochondria. J. Cell Biol 30:269
    [Google Scholar]
  6. Hanson J. B., Stoner C. D., Hodges T. K. 1964; Chloramphenicol as an inhibitor of energy-linked processes in maize mitochondria. Nature, Lond 203:258
    [Google Scholar]
  7. Hendrix J. W., Lauder D. K. 1966; Effects of polyene antibiotics on growth and sterol-induc- tion of oospore formation by Pythium periplocum . J. gen. Microbiol 44:115
    [Google Scholar]
  8. Hess W. M. 1966; Fixation and staining of fungus hyphae and host plant root tissues for electron microscopy. Stain Technol 41:27
    [Google Scholar]
  9. Huang M., Biggs D. R., Clark-Walker G. D., Linnane A. W. 1966; Chloramphenicol inhibition of the formation of particulate mitochondrial enzymes of Saccharomyces cerevisiae . Biochim. biophys. Acta 114:434
    [Google Scholar]
  10. Johansen D. E. 1940 Plant Microtechnique, 1. New York: McGraw-Hill;
    [Google Scholar]
  11. Kroon A. M. 1963; Protein synthesis in heart mitochondria I. Amino acid incorporation into the protein of isolated beef-heart mitochondria and fractions derived from them by sonic oscillation. Biochim. biophys. Acta 72:391
    [Google Scholar]
  12. Mager J. 1960; Chloramphenicol and chlortetracycline inhibition of amino acid incorporation into proteins in a cell-free system from Tetrahymena pyriformis . Biochim. biophys. Acta 38:150
    [Google Scholar]
  13. Marchant R., Peat A., Banbury G. H. 1967; The ultrastructural basis of hyphal growth. New Phytol 66:623
    [Google Scholar]
  14. Morris I. 1966a; Some effects of chloramphenicol on the metabolism of Chlorella. I. The effect on protein, polysaccharide and nucleic acid synthesis. Arch. Mikrobiol 54:160
    [Google Scholar]
  15. Morris I. 1966b; Some effects of chloramphenicol on the metabolism of Chlorella. II. The effect on permeability and on the internal concentration of adenosine triphosphate (ATP). Arch. Mikrobiol 54:169
    [Google Scholar]
  16. Parsons D. F. 1963; Mitochondrial structure: two types of subunits on negatively stained mitochondrial membranes. Science, N. Y 140:985
    [Google Scholar]
  17. Racker E., Chance B., Parsons D. F. 1964; Correlation of structure and function of sub- mitochondrial units in oxidative phosphorylation. Fedn. Proc 23431
    [Google Scholar]
  18. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol 17:208
    [Google Scholar]
  19. So A. G., Da vie E. W. 1963; The incorporation of amino acids into protein in a cell-free system from yeast. Biochemistry, N.Y 2:132
    [Google Scholar]
  20. Stasny J. T., Crane F. L. 1964; The effect of sonic oscillation on the structure and function of beef heart mitochondria. J. Cell Biol 22:49
    [Google Scholar]
  21. Vazquez D. 1966; The mode of action of chloramphenicol and related antibiotics. Symp. Soc.gen. Microbiol 16169
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-50-3-391
Loading
/content/journal/micro/10.1099/00221287-50-3-391
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error