The Chemical Composition of the Nucleic Acids and the Proteins of Some Mycoplasma Strains Free

Abstract

SUMMARY

The base compositions of the deoxyribonucleic acid (DNA) and ribo-nucleic acid (RNA) of three strains of and one of var. have been determined. The adenine (A) + thymine (T) content of all the samples examined was much greater than the guanine (G) + cytosine (C) content, the values for % GC ranging from 34·4 to 32·5 for the sample of to 30·0 for var. . The adenine + uracil/guanine + cytosine value for the RNA of all the samples was in the range 1·17–1·20.

The amino acid content of the protein of was also determined. This agrees closely with the values obtained by Sueoka (1961) for the amino acid composition of the protein for a bacterium having the same DNA base composition.

The values given in a previous communication (Jones, Tittensor & Walker, 1965) for the amino acid composition of the protein of var. were incorrect since a protein fraction had precipitated from the medium during the growth of the organism because of the decrease in pH value of the culture which occurred. The correct values are now given and they agree closely with the values predicted from the work of Sueoka (1961).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-50-2-305
1968-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/50/2/mic-50-2-305.html?itemId=/content/journal/micro/10.1099/00221287-50-2-305&mimeType=html&fmt=ahah

References

  1. Chelton E. T. J. 1963; Preventing temperature rise during the mechanical breakage of microorganisms. J. appl. Bact 26:178
    [Google Scholar]
  2. Hill L. R. 1966; An index to deoxyribonucleic acid base compositions of bacterial species. J. gen. Microbiol 44:419
    [Google Scholar]
  3. Jones A. S., Tittensor J. R., Walker R. T. 1965; The chemical composition of the nucleic acids and other macromolecular constituents of Mycoplasma mycoides var. capri. J. gen. Microbiol 40:405
    [Google Scholar]
  4. Kolbe J. J. 1960; Avoidance of alkaline effects in cell disruption by glass beads. Biochim. biophys. Acta 38:373
    [Google Scholar]
  5. Lynn R. J., Smith P. F. 1957; Nucleic acid content of pleuropneumonia-like organisms from human sources. J. Bact 74:811
    [Google Scholar]
  6. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. molec. Biol 5:109
    [Google Scholar]
  7. Morowitz H. J., Tourtellotte M. E., Guild W. R., Castro E., Woese C., Cleverdon R. C. 1962; The chemical composition and sub-microscopic morphology of Mycoplasma gallisepticum Avian PPLO 5969. J. molec. Biol 4:93
    [Google Scholar]
  8. Neimark H. C., Pène J. J. 1965; Characterization of pleuropneumonia-like organisms by deoxyribonucleic acid composition. Proc. Soc. exp. Biol. Med 118:517
    [Google Scholar]
  9. Rogul M., McGee Z. A., Wittler R. G., Falkow S. 1965; Nucleic acid homologies of selected bacterial, L. forms and Mycoplasma species. J. Bact 90:1200
    [Google Scholar]
  10. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. molec. Biol 4:430
    [Google Scholar]
  11. Sueoka N. 1961; Correlation between base composition of deoxyribonucleic acid and amino acid composition of protein. Proc. natn. Acad. SciU.S.A. 471141
    [Google Scholar]
  12. Wyatt G. R., Cohen S. S. 1953; The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethyl cytosine. Biochem. J 55:774
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-50-2-305
Loading
/content/journal/micro/10.1099/00221287-50-2-305
Loading

Data & Media loading...

Most cited Most Cited RSS feed