Study of a Small Amoeba from Mammalian Cell Cultures Infected with ‘Ryan Virus’ Free

Abstract

SUMMARY

A small amoeboid organism, found in mammalian tissue cultures inoculated with an infective agent formerly termed ‘Ryan virus’, is shown to have the morphological, cultural and behavioural characters of the free-living soil amoeba Douglas, 1930. Cytopathic changes occurred regularly in the infected monolayers; this was evidently due to action of the amoebae rather than the presence of any associated bacterial or viral agents. Strong circumstantial evidence suggests that the Ryan isolates of originated, either as trophozoites or cysts, from swabs of the human nasopharynx. Recovery of hartmannellid amoebae from this source is of interest in relation to some recently reported cases of pyogenic meningitis, apparently caused by free-living soil amoebae.

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1968-02-01
2024-03-28
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References

  1. Adam K. M. G. 1964; A comparative study of Hartmannellid amoebae. J. Protozool 11:423
    [Google Scholar]
  2. Alexeieff A. 1912a; Sur les caractéres cytologiques et la systematique des Amibes du groupe Limax. Bull. Soc. zool. Fr 37:55
    [Google Scholar]
  3. Alexeieff A. 1912b; Quelques remarques supplémentaires sur la systématique des Amibes du groupe Limax. Sur le genre Sappina Dangeard. Bull. Soc. zool. Fr 37:149
    [Google Scholar]
  4. Armstrong J. A., Pereira M. S. 1967; Indemnification of ‘Ryan Virus’ as an amoeba of the genus Hartmannella. Br. med. J i:212
    [Google Scholar]
  5. Butt C. G. 1966; Primary amebic meningoencephalitis. New Engl. J. Med 274:1473
    [Google Scholar]
  6. Castellani A. 1930a; An amoeba found in cultures of a yeast. Preliminary note. J. trop. Med. Hyg 33:160
    [Google Scholar]
  7. Castellani A. 1930b; An amoeba growing in cultures of a yeast. Second note. J. trop. Med. Hyg 33:188
    [Google Scholar]
  8. Castellani A. 1930c; An amoeba found in cultures of a yeast. Third note. J. trop. Med. Hyg 33:221
    [Google Scholar]
  9. Castellani A. 1930d; An amoeba growing in cultures of a yeast. Fourth note. J. trop. Med. Hyg 33:237
    [Google Scholar]
  10. Chang R. S. 1961; Properties of a transmissible agent capable of inducing marked DNA degradation and thymine catabolism in a human cell. Proc. Soc. exp. Biol. Med 107135
    [Google Scholar]
  11. Chang R. S., Hung Pan I., Rosenau B. J. 1966; On the nature of the ‘Lipovirus’. J. exp. Med 124:1153
    [Google Scholar]
  12. Culbertson C. G. 1961; Pathogenic Acanthamoeba. Am. J. clin. Path 35:195
    [Google Scholar]
  13. Culbertson C. G., Smith J. W., Minner J. R. 1958; Acanthamoeba; observations on animal pathogenicity. Science, N.Y 127:1506
    [Google Scholar]
  14. Culbertson C. G., Smith J. W., Cohen H. K., Minner J. R. 1959; Experimental infection of mice and monkeys by Acanthamoeba. Am. J. Path 35:185
    [Google Scholar]
  15. Douglas M. 1930; Notes on the classification of the amoeba found by Castellani in cultures of a yeast-like fungus. J. trop. Med. Hyg 33:258
    [Google Scholar]
  16. Eldridge A. E., Tobin J. O’H. 1967; ‘Ryan Virus’. Br. med. J i:299
    [Google Scholar]
  17. Fowler M., Carter R. F. 1965; Acute pyogenic meningitis probably due to Acanthamoeba sp. A preliminary report. Br. med. J ii:740
    [Google Scholar]
  18. Jahnes W. G., Fullmer H. M., Li C. P. 1957; Free living amoebae as contaminants in monkey kidney tissue culture. Proc. Soc. exp. Biol. Med 96484
    [Google Scholar]
  19. Neff R. J. 1957; Purification, axenic cultivation, and description of a soil amoeba, Acanthamoeba sp. J. Protozool 4:176
    [Google Scholar]
  20. Pereira M. S., Marsden H. B., Corbitt G., Tobin J., O’H. 1966; Ryan Virus; a possible new human pathogen. Br. med. J i:130
    [Google Scholar]
  21. Singh B. N. 1950; A culture method for growing small free-living amoebae for the study of their nuclear division. Nature, Lond 165:65
    [Google Scholar]
  22. Singh B. N. 1952; Nuclear division in nine species of small free-living amoebae and its bearing on the classification of the order Amoebida. Phil. Trans. R. Soc. B 236:405
    [Google Scholar]
  23. van Rooyen C. E. 1932a; The effect of Amoeba (Hartmannella) castellanii on bacterial cultures. J. trop. Med. Hyg 35:118
    [Google Scholar]
  24. van Rooyen C. E. 1932b; Observations on the clearing effect of Amoeba (Hartmannella) castellanii on bacterial cultures: a phenomenon simulating bacteriophagy. J. trop. Med. Hyg 35:259
    [Google Scholar]
  25. Volkonsky M. 1931; Hartmannella castellanii Douglas et classification des Hartmannelles. Archs Zool. exp. gen 72:317
    [Google Scholar]
  26. Wang S. S., Feldman H. A. 1961; Occurrence of Acanthamoeba in tissue cultures inoculated with human pharyngeal swabs. In Antimicrobial Agents and Chemotherapy 150 Finland M., Savage G. M. Michigan: Braun-Brumfield Inc;
    [Google Scholar]
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