1887

Abstract

SUMMARY

The aerobic growth of adapted to a chemically defined glucose ammonium sulphate medium was studied in the same medium containing triethylene-melamine (TEM). In liquid medium containing TEM up to 205 mg./l. growth and division were only slightly retarded. With concentrations of TEM greater than about 255 mg./l. division was strongly inhibited while growth continued slowly, giving filaments the longest of which later separated from the medium as a white pellicle. Further growth and division occurred in the same culture after a lag. During continued subculture in liquid medium containing a constant concentration of TEM the lag was negligible but growth on return to drug-free liquid or solid medium was impaired. At first filament formation increased to a maximum, the growth rate fluctuated and adaptation was rapidly lost during subculture in drug-free medium. The progressive impairment of division was later opposed by a gradual adaptation which eventually produced bacteria of a more nearly normal size, with a steady growth rate and with resistance to precipitation and to higher concentrations of TEM. TEM was less active in solid medium than in liquid medium. The proportion of filaments and the size of the lenticular areas observed in colonies increased with increasing concentration of TEM but decreased with increasing age and size of the colonies.

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1968-01-01
2022-01-18
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References

  1. Davies D. S., Hinshelwood C. N., Pryce J. M. G. 1944; Studies in the mechanism of bacterial adaptation. Trans. Faraday Soc 40:397
    [Google Scholar]
  2. Dean A. C. R., Hinshelwood Sir C. 1955; The rate of development of colonies of Bacterium lactis aerogenes on agar plates containing drugs. Proc. R. Soc. B 144297
    [Google Scholar]
  3. Dean A. C. R., Hinshelwood Sir C. 1957a; The formation of papillae on bacterial colonies I. Proc. R. Soc. B 1471
    [Google Scholar]
  4. Dean A. C. R., Hinshelwood Sir C. 1957b; The formation of papillae on bacterial colonies 11. Proc. R. Soc. B 14710
    [Google Scholar]
  5. Dean A. C. R., Hinshelwood Sir C. 1966 Growth, Function and Regulation in Bacterial Cells Oxford: Clarendon Press;
    [Google Scholar]
  6. Drabble W. T., Hinshelwood Sir C. 1961; Development of resistance to streptomycin by Bact. lactis aerogenes (Aerobacter aerogenes). 1. The role of mutation and of physiological adaptation. Proc. R. Soc. B 154449
    [Google Scholar]
  7. Grant D. J. W. 1967; Kinetic aspects of the growth of Aerobacter aerogenes with some benzenoid carbon sources. J. gen. Microbiol 46:213
    [Google Scholar]
  8. Hinshelwood C. N., Lodge R. M. 1944; A physico-chemical study of some induced changes in the morphology of Bacterium lactis aerogenes A theory of the balance and adaptive variation of certain enzyme processes in bacteria. Proc. R. Soc. B 13247
    [Google Scholar]
  9. Iyer Y. N., Szybalski W. 1958; Two simple methods for the detection of chemical mutagene. Appl. Microbiol 6:23
    [Google Scholar]
  10. Katchman B. J., Spoerl E., Smith H. E. 1955; Effects of cell division inhibition on phosphorus metabolism of Escherichia coli . Science 121:97
    [Google Scholar]
  11. Kilgore W. W., Greenberg J. 1961; Filament formation and resistance to 1-methyl-3-nitro-1-nitrosoguanidine and other radiometric compounds in Escherichia coli . J. Bact 81:258
    [Google Scholar]
  12. Loveless L. E., Spoerl E., Weisman T. H. 1954; A survey of effects of chemicals on division and growth of yeast and Escherichia coli . J. Bact 68:637
    [Google Scholar]
  13. Maxwell R. E., Nickel V. S. 1954; Filament formation in E. coli induced by azaserine and other antineoplastic agents. Science 120:270
    [Google Scholar]
  14. Monod J. 1942 Recherches sur la Croissance des Cultures Bactériennes Paris: Hermann et Cie;
    [Google Scholar]
  15. Poole A., Hinshelwood C. N. 1940; Physico-chemical aspects of bacterial growth. Part VI. The influence of toxic substances on growth rate, stationary population and fermentation reactions of Bact. lactis aerogenes . J. chem. Soc1565
    [Google Scholar]
  16. Schweisfurth R. 1959; Einwirkung einiger Gifte auf Zeli- und Nucleoid-teilung bei Bakterien. Zentbl. Bakt. ParasitKde, Abt. 11 113:48
    [Google Scholar]
  17. Spray G. H., Lodge R. M. 1943; The effects of resorcinol and m-cresol on the growth of Bact. lactis aerogenes . Trans. Faraday Soc 39:424
    [Google Scholar]
  18. Szybalski W. 1960; The mechanism of chemical mutagenesis with special reference to triethylene melamine action. Developments in Industrial Microbiology 1:231
    [Google Scholar]
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