Nutritional Requirements of Bacterium coli at 44°

Summary: A strain of Bacterium coli capable of growth in broth and in an ammonium + glucose + salts medium at 37° and in broth at 44°, died in the defined medium at 44°. The addition of glutamic acid to this medium allowed the growth temperature to be raised slightly; the further addition of nicotinamide allowed a further increase in growth temperature.