1887

Abstract

SUMMARY: The role of electrolytes in the adsorption of the citrate-sensitive staphylococcal typing-phages and the citrate-insensitive phage K on to their propagating strains was studied in broth+Na citrate and in distilled water+CaCl, MgCl, and NaCl. Some phages when in the free state were partially inactivated by 1% (w/v) Na citrate. In all except two phages, K and 51, citrate inhibited adsorption in broth; adsorption occurred in distilled water. Three phages, 7, 42B and 42E, had a specific requirement of 50 g. CaCl/ml. for adsorption. With the remainder, the requirements of CaCl varied from 5 to 400 g./ml.; similar amounts of MgCl acted equally well.

With the citrate-sensitive phages adsorption in NaCl was variable and, where it occurred, recovery of viable phage from cells infected in NaCl was less than from cells infected in CaCl. These phages appear to have a specific requirement of divalent cations for adsorption, suggesting that adsorption and penetration are enzymic in character.

The electrolyte requirements of the citrate-insensitive phage K were related geometrically to the valence of the cations used, 5 g. CaCl or MgCl/ml, or 25 g. NaCl/ml. giving optimum adsorption.

Erratum

An erratum has been published for this content:
CORRIGENDA (Volume 5)
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-5-4-673
1951-12-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/5/4/mic-5-4-673.html?itemId=/content/journal/micro/10.1099/00221287-5-4-673&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1949; The calcium requirement of coli phage T5. J. Immunol 62:505
    [Google Scholar]
  2. Anderson T. F. 1945; The role of tryptophane in the adsorption of two bacterial viruses on their host, E. coli. J. cell. & comp. Phys 25:17
    [Google Scholar]
  3. Burnet F. M. 1933; The classification of dysentery-coli bacteriophages. III. A correlation of the serological classification with certain biochemical tests. J. Path. Bact 37:179
    [Google Scholar]
  4. Burnet F. M., Lush D. 1935; The staphylococcal bacteriophages. J. Path. Bact 40:455
    [Google Scholar]
  5. Cohen S. S. 1949; Growth requirements of bacterial viruses. Bact. Rev 13:1
    [Google Scholar]
  6. Delbrück M. 1948; Biochemical mutants of bacterial viruses. J. Bact 56:1
    [Google Scholar]
  7. Edney M. 1949; The influence of calcium ion on the reactions of the V. cholerae enzyme R.D.E. and influenza virus with specific mucopolysaccharides. Aust. J. exp. Biol. med. Sci 27:252
    [Google Scholar]
  8. Elford W. J., Andrewes C. H. 1932; The ‘killing’ of bacteria by bacteriophage. Brit. J. exp. Path 13:13
    [Google Scholar]
  9. Puck T. T. 1949; A reversible transformation of Tl bacteriophage. J. Bact 57:647
    [Google Scholar]
  10. Rountree P. M. 1947; Staphylococcal bacteriophages. II. Bacteriophage absorption by staphylococci. Aust. J. exp. Biol. med. Sci 25:203
    [Google Scholar]
  11. Rountree P. M. 1949; The serological differentiation of staphylococcal bacteriophages. J. gen. Microbiol 3:164
    [Google Scholar]
  12. Williams Smith H. 1948; Calcium-deficient media: their effect on phage action. Nature; Lond: 161397
    [Google Scholar]
  13. Wilson G. S., Atkinson J. D. 1945; Typing of staphylococci by the bacteriophage method. Lancet i:647
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-5-4-673
Loading
/content/journal/micro/10.1099/00221287-5-4-673
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error