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The heat-stability of the T antigen of strains of Streptococcus pyogenes grown in Pope &Smith (1932) broth (containing maltose and yeast) with added pancreatic extract varied considerably from strain to strain; with several strains 30 minutes' boiling was insufficient to eliminate all T agglutinability.
By heating suspensions of streptococci to 80 for 30 min. and then treating the heated suspensions with trypsin-containing pancreatic extract for a few hours, T antigen was obtained in solution and could be demonstrated by precipitin tests with appropriate type-specific sera.
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