1887

Abstract

SUMMARY: Three initially streptomycin-sensitive strains of Mycobacterium tuber-cuZosis, two from patients with tuberculosis, and one the laboratory strain H37Rv, were grown for 6 weeks on Herrold s agar medium containing graded concentrations of streptomycin. Discrete colonies of s that grew on the streptomycin media were tested for streptomycin sensitivity in Tween 80 albumin medium.

In all three strains the distribution of resistance was discontinuous, the subcultures from the colonies being either sensitive, or resistant to streptomycin in three distinct ranges of minimal inhibitory concentrations, namely, 0·25–0·5,1–2 and 32–64, g./ml.; and of initial populations of 0·4–1·0 × 10 bacilli, about 1000 were in the first, slightly resistant group, about 100 in the second, and about 2 were in the third, highly resistant group.

Of the two patients from whom the two of the strains were isolated, one developed a highly resistant strain in his sputum during treatment, whereas the other developed a strain only of slight resistance, in spite of prolonged streptomycin treatment.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-5-3-596
1951-08-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/5/3/mic-5-3-596.html?itemId=/content/journal/micro/10.1099/00221287-5-3-596&mimeType=html&fmt=ahah

References

  1. Gots J. S., Sevag M. G. 1948; Enzymatic studies on the mechanism of resistance of pneumococci to drugs. III. Experimental results indicating alteration in enzyme proteins associated with the development of resistance to drugs. J. Bact 56:737
    [Google Scholar]
  2. Maas E. A., Johnson M. J. 1949; Penicillin uptake by bacterial cells. J. Bact 57:415
    [Google Scholar]
  3. Meads M., Haslam N. H. 1949; Quantitative studies on the origin and characteristics of streptomycin-fast variants of Klebsielle pneumoniae. J. Immunol 63:1
    [Google Scholar]
  4. Medical Research Council 1948; Specific laboratory tests in streptomycin therapy of tuberculosis. Lancet ii862
    [Google Scholar]
  5. Mitchison D. A. 1949; Tests for streptomycin sensitivity of tubercle bacilli in Tween 80 albumin liquid medium. Lancet ii:694
    [Google Scholar]
  6. Oginsky E. L., Smith P. H., Umbreit W. W. 1949; The action of streptomycin. 1. The nature of the reaction inhibited. J. Bact 58:747
    [Google Scholar]
  7. Pyle M. M. 1947; Relative numbers of resistant tubercle bacilli in sputa of patients treated with streptomycin. Proc. Mayo Clin 22:465
    [Google Scholar]
  8. Stamp Lord. 1947; The preservation of bacteria, by drying. J. gen. Microbiol 1:251
    [Google Scholar]
  9. Vennesland K., Ebert R., Bloch R. 1947; The demonstration of naturally occurring streptomycinTresistant variants in the human strain of tubercle bacillus H37Rv. Science 106:476
    [Google Scholar]
  10. Yegian D., Vanderlinde R. J. 1948; A quantitative analysis of the resistance of mycobacteria to streptomycin. J. Bact 56:177
    [Google Scholar]
  11. Youmans A. S., Youmans G. P. 1948; Effect of ‘Tween 80’ in vitro on bacteriostatic activity of 20 compounds for Myco. tuberculosis. J. Bact 52:245
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-5-3-596
Loading
/content/journal/micro/10.1099/00221287-5-3-596
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error