1887

Abstract

SUMMARY: Representative strains of the pleuropneumonia group, originally isolated from animals, grew well on a basal medium containing infusion broth, peptone, yeast extract and agar when this was further enriched with horse serum. The serum could be replaced by an ethereal extract of egg-yolk and fractionation of this suggested that cholesterol might be the active substance promoting growth. Growth was obtained when cholesterol (0·01 mg./ml.) was added to the basal medium, together with starch or bovine albumin. Addition of the acetone-insoluble fraction of egg-yolk with cholesterol gave better results, growth of six of the eight strains tested being equal to that on serum agar; the lecithin and kephalin fractions after purification were less effective. No growth was obtained when starch, bovine albumin or the acetone-insoluble lipid was added to the medium without cholesterol. Cholestanol and stigmasterol were as effective as cholesterol in promoting growth. Oleic acid in high concentrations (> 0.05 mg./ml.) inhibited growth. Both lipid and protein fractions of serum appeared to be concerned in its ability to promote growth.

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/content/journal/micro/10.1099/00221287-5-3-576
1951-08-01
2022-08-13
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References

  1. Bloor W. R. 1943 Biochemistry of the Fatty Acids and their Compounds, the Lipids New York: Reinhold Publishing Corporation;
    [Google Scholar]
  2. Cailleau R. 1937; La nutrition des flagellés tétramitidés. Les sterols, facteurs de croissance pour les trichomonades. Ann. Inst. Pasteur 59:293
    [Google Scholar]
  3. Davis B. D., Dubos R. J. 1947; .The binding of fatty acids by serum albumin, a protective growth factor in bacteriological media. J. exp. Med 86:215
    [Google Scholar]
  4. Dienes L. 1950; Isolation of L type cultures from Clostridia. Proc. Soc. exp. Biol., N.Y 75:412
    [Google Scholar]
  5. Edward D. G. FF. 1947; A selective medium for pleuropneumonia-like organisms. J. gen. Microbiol 1:238
    [Google Scholar]
  6. Edward D. G. FF. 1950; An investigation of the biological properties of organisms of the pleuropneumonia group, with suggestions regarding the identification of strains. J. gen. Microbiol 4:311
    [Google Scholar]
  7. Edward D. G. FF., Fitzgerald W. A. 1951; The isolation of organisms of the pleuropneumonia group from dogs. J. gen. Microbiol 5:566
    [Google Scholar]
  8. Griffin A. M., McCartin W. G. 1949; Sterols and fatty acids in the nutrition of entozoic amoebae in cultures. Proc. Soc. exp. Biol., N.Y 72:645
    [Google Scholar]
  9. Hartley P. 1925; Observations on the role of the ether-soluble constituents of serum in certain serological reactions. Brit. J. exp. Path 6:180
    [Google Scholar]
  10. Haslewood G. A. D. 1944; Cholesterol metabolism in the animal body. Nature; Lond: 15429
    [Google Scholar]
  11. Laidlaw P. P., Elford W. J. 1936; A new group of filtrable organisms. Proc. roy. Soc. B 120:292
    [Google Scholar]
  12. Lwoff A. 1947; Sur le role du serum dans le développement de Moraxella lacunata et du Neisseria gonnorrhae. Ann. Inst. Pasteur 73:735
    [Google Scholar]
  13. McKinnis H.jun 1947; The growth-promoting activity of cholesterol and related compounds in black carpet beetle larvae. J. biol. Chem 167:645
    [Google Scholar]
  14. Partridge S. M., Klieneberger E. 1941; Isolation of cholesterol from the oily droplets found in association with the L1 organism separated from Streptobacillus moniliformis. J. Path. Bact 52:219
    [Google Scholar]
  15. Pollock M. R. 1949; The effects of long-chain fatty acids on the growth of Haemophilus pertussis and other organisms. Symposia of the Society for Experimental Biology3 p. 193 Cambridge University Press;
    [Google Scholar]
  16. Sabin A. B. 1941; The filtrable micro-organisms of the pleuropneumonia group. Bact. Rev 5:1
    [Google Scholar]
  17. Sobel H., Plaut A. 1949; The assimilation of cholesterol by Mycobacterium smegmatis. J. Bact 57:377
    [Google Scholar]
  18. Turfitt G. E. 1947; Microbiological agencies in the degradation of steroids. II. Steroid utilization by the microflora of soils. J. Bact 54:557
    [Google Scholar]
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