SUMMARY: A modification of the Hirst & Pickels photoelectric densitometer and its application to work on influenza virus haemagglutination are described. The methods used are believed to reduce to a minimum the inherent margin of error of virus and serum antibody assays based upon the haemagglutination reaction. In order to make full use of data thus obtained precise assessment of the significance of differences of titre is essential, and for this purpose two simple statistical tests have been applied and their application illustrated by an example.


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