1887

Abstract

SUMMARY: For maximal zones of ‘exhibition’ in the plate test for vitamin B with Dorner ATCC 8000, using the media described, the presence of both oxygen and vitamin B is necessary. Under anaerobic conditions the vitamin is not an essential growth factor and under highly aerobic conditions growth does not occur even in its presence. Under the conditions of maintenance used variant strains were not detected.

When grown in the presence of vitamin B the bacteria may store sufficient of the vitamin to render measurements of growth useless for assay purposes: when grown under anaerobic conditions the culture obtained is unsuitable for use as an inoculum. A technique for preparing satisfactory inocula of the test organisms and the conditions necessary for well-defined responses to vitamin B are described.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-5-2-416
1951-05-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/5/2/mic-5-2-416.html?itemId=/content/journal/micro/10.1099/00221287-5-2-416&mimeType=html&fmt=ahah

References

  1. Bacharach A. L., Cuthbertson W. F. J. 1948; The cup-plate method in microbiological assay with special reference to riboflavine and aneurine. Analyst 73:334
    [Google Scholar]
  2. Cuthbertson W. F. J. 1949; Estimation of the anti-pernicious anaemia factor. Biochem. J 44:v
    [Google Scholar]
  3. Cuthbertson W. F. J., Lloyd Joan T., Pegler H. F. 1951; The assay of vitamin B12. Part III. Microbiological estimation with Lactobacillus lactis Domer by the plate method. Analyst 76:133
    [Google Scholar]
  4. Foster J. C., Lally J. A., Woodruff H. B. 1949; Cup assay with vitamin B12 as a standard. Science 110:507
    [Google Scholar]
  5. Greene R. D., Brook A. J., McCormack R. B. 1949; Some conditions which affect the assay of vitamin B12 with Lactobacillus lactis Dorner. J. biol. Chem 178:999
    [Google Scholar]
  6. Koditschek L. K., Hendlin D., Woodruff H. B. 1949; Investigations on the nutrition of Lactobacillus lactis Dorner. J. biol. Chem 179:1093
    [Google Scholar]
  7. Pontecorvo G. 1949; Auxanographic techniques in biochemical genetics. J. gen. Microbiol 3:122
    [Google Scholar]
  8. Roberts E. C., Snell E. E. 1946; An improved medium for microbiological assay with Lactobacillus casei. J. biol. Chem 163:499
    [Google Scholar]
  9. Shorb M. S., Briggs G. M. 1948; The effect of dissociation in Lactobacillus lactis cultures on the requirement for vitamin B12. J. biol. Chem 176:1463
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-5-2-416
Loading
/content/journal/micro/10.1099/00221287-5-2-416
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error