SUMMARY: For maximal zones of ‘exhibition’ in the plate test for vitamin B with Dorner ATCC 8000, using the media described, the presence of both oxygen and vitamin B is necessary. Under anaerobic conditions the vitamin is not an essential growth factor and under highly aerobic conditions growth does not occur even in its presence. Under the conditions of maintenance used variant strains were not detected.

When grown in the presence of vitamin B the bacteria may store sufficient of the vitamin to render measurements of growth useless for assay purposes: when grown under anaerobic conditions the culture obtained is unsuitable for use as an inoculum. A technique for preparing satisfactory inocula of the test organisms and the conditions necessary for well-defined responses to vitamin B are described.


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