SUMMARY: No significant improvement has been made on the medium first described for the primary culture of by Bordet&Gengou in 1906. An explanation for this was sought in the experiments described, and it was found to be most probable that potato extract provides optimal concentrations of amino-acids and peptides required for growth. Although serum can be used to replace whole blood in this medium, the latter is essential for maximal growth; for that reason the retention of the term haemophilus is justified.

Many peptones, especially the more digested ones, inhibit growth of in concentrations normally used in bacteriological media. In the absence of peptone, meat extract agar can be used as the base of a satisfactory medium, especially when used in conjunction with horse instead of rabbit blood.


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