1887

Abstract

SUMMARY: Freshly isolated strains of vary considerably in their virulence for mice when tested by the intranasal route; this variation is continous and must be regarded as a quantitative not a qualitative character. In order to titrate virulence it is necessary to achieve and maintain a standard technique with cultures and to use ‘standard’ mice kept under standardized conditions. It was not possible to show a correlation between virulence (as measured by the LD50) and either the viable count or the presence of toxin of haemagglutinin in the suspensions used in the virulence test. Nor was it possible to show any correlation between virulence and the ‘age’ of the culture, either in number of subcultures, time elapsing between swabbing and testing, type of swab or duration of illness (up to three weeks) when the swab was taken. Some strains kill mice more quickly than others; this ability is attributed to a ‘quick-killing factor’.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-5-2-250
1951-05-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/5/2/mic-5-2-250.html?itemId=/content/journal/micro/10.1099/00221287-5-2-250&mimeType=html&fmt=ahah

References

  1. Anderson G., North E. A. 1943; The relationship of pertussis endotoxin to pertussis immunity in the mouse. Aust. J. exp. Biol. med. Sci 21:1
    [Google Scholar]
  2. Burnet F. M., Timmins C. 1937; Experimental infection with Haemophilus pertussis in the mouse by intranasal inoculation. Brit. J. exp. Path 18:83
    [Google Scholar]
  3. Cruickshank J. C., Freeman G. G. 1937; Immunizing fractions isolated from Haemophilus pertussis. Lancet ii:567
    [Google Scholar]
  4. Culotta C. A., Martin F. L., Liebow A. A. 1938; Whooping Cough: observations on experimental infection in mice and on attempts at active immunization in mice and in ferrets. Yale J. Biol. Med 10:233
    [Google Scholar]
  5. Elderling G. 1942; A study of the antigenic properties of H. pertussis and related organisms. II. Protection tests in mice. Amer. J. Hyg 36:294
    [Google Scholar]
  6. Evans D. 1949; Private communication.
    [Google Scholar]
  7. Gray D. F. 1946; Some factors influencing the virulence of Haemophilus pertussis Phase I. Aust. J. exp. Biol. med. Sci 24:301
    [Google Scholar]
  8. Hoyle L., Orr J. W. 1945; The histogenesis of experimental pneumonics in mice. J. Path. Bact 57:441
    [Google Scholar]
  9. Irwin J. O. 1951; Note on the relation between LD50 and average time of death in 25 strains of H. pertussis. J. gen. Microbiol 5:266
    [Google Scholar]
  10. Kendrick P. L., Elderling G., Dixon M. K., Misner J. 1947; Mouse protection tests in the study of pertussis vaccines. Amer. J. publ. Hlth 37:803
    [Google Scholar]
  11. Keogh E. V., North E. A. 1948; The haemagglutinin of Haemophilus pertussis. I. Haemagglutinin as a protective antigen in experimental murine pertussis. Aust. J. exp. Biol. med. Sci 26:315
    [Google Scholar]
  12. Lapin J. H. 1943 Whooping Cough Springfield, III, U.S.A: Chas. C. Thomas;
    [Google Scholar]
  13. Leslie P. H., Gardner A. D. 1931; The phases of H. pertussis. J. Hyg., Camb 31:423
    [Google Scholar]
  14. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 38:732
    [Google Scholar]
  15. Norton J. F., Dingle J. H. 1935; Virulence tests for typhoid bacilli and antibody relationships in antityphoid sera. Amer. J. publ. Hlth 37:803
    [Google Scholar]
  16. Proom H. 1947; The immunological aspects of experimental Haemophilus pertussis infection. J. Path. Bact 59:165
    [Google Scholar]
  17. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. Amer. J. Hyg 27:493
    [Google Scholar]
  18. Strean L. P. 1943; Attempts to enhance the virulence of H. pertussis by serial passage in mice. Amer. J. Dis. Child 65:895
    [Google Scholar]
  19. Toomey J. A., Ranta K., Takacs W. S. 1935; The virulence of H. pertussis. J. infect. Dis 57:286
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-5-2-250
Loading
/content/journal/micro/10.1099/00221287-5-2-250
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error