New Techniques for the Study of Growin? Micro-or?anisms Free

Abstract

SUMMARY: A method is described for the examination of growing micro-organisms by vertical and oblique incident illumination using an illuminator of the ‘Universal Illuminator’ (Messrs Cooke, Troughton and Simms) type. A moist chamber has been developed to enable this method to be used for the routine examination of bacterial cultures, and examples are given of both aerobic and anaerobic cultures on the surface of solid nutrient media, preferably containing nigrosine.

This method has also been used for following spore germination of in a liquid medium. A method using Cellophane as a support for growth is also described. Certain organisms can be grown on Cellophane ‘windows’ made in the caps of Bijou bottles containing a liquid medium; this apparatus is suitable for the repeated but not continuous examination of young growths.

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/content/journal/micro/10.1099/00221287-5-1-91
1951-02-01
2024-03-29
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References

  1. Barnes J. M. 1947; The development of anthrax following the administration of spores by inhalation. Brit. J. exp. Path 28:385
    [Google Scholar]
  2. Carpenter W. B. 1891 The microscope and its revelations, 7th ed. Dallinger W. H. London: J. and A. Churchill;
    [Google Scholar]
  3. Conway E. J., Byrne A. 1933; An absorption apparatus for the micro-determination of certain volatile substances. I. The micro-determination of ammonia. Biochem. J 27:419
    [Google Scholar]
  4. Gladstone G. P. 1948; Immunity to anthrax. Production of the cell-free protective antigen in Cellophane sacs. Brit. J. exp. Path 29:379
    [Google Scholar]
  5. Gladstone G. P., Fildes P. 1940; A simple medium for general use without meat extract or peptone. Brit. J. exp. Path 21:161
    [Google Scholar]
  6. Graham-Smith G. S. 1910; The division and post-fission movements of bacilli when grown on solid media. Parasitology 3:17
    [Google Scholar]
  7. Hills G. M., Belton F. C., Blatchley, Errol D. 1949; Ayfivin: production in chemically defined media and comparison with licheniformin. Brit. J. exp. Path 30:427
    [Google Scholar]
  8. Klieneberger E., Smiles J. 1942; Some new observations on the developmental cycle of bovine pleuropneumonia and related microbes. J. Hyg., Camb 42:110
    [Google Scholar]
  9. Knaysi G. 1944 Elements of Bacterial Cytology Ithaca, N.Y., U.S.A.: Comstock Publishing Co;
    [Google Scholar]
  10. Langeron M. 1948 Précis de Microscopie, 7th ed. Paris: Masson et Cie;
    [Google Scholar]
  11. Madras S., Mason S. G., McIntosh R. L. 1949; A preliminary study of the permeability of Cellophane to liquids. Canad. J. Res 27, (B):764
    [Google Scholar]
  12. McKercher D. G., Reed G. B. 1948; Surface growth of bacteria on Cellophane. Canad. J. Res 26, (E):330
    [Google Scholar]
  13. Stitt E. R., Clough P. W., Clough M. C. 1938 Practical Bacteriology, Haematology, and Animal Parasitology, 9th ed. Philadelphia, Pa., U.S. A: The Blakiston Company;
    [Google Scholar]
  14. Topley and Wilson’s Principles of Bacteriology and Immunity 1946, 3rd. ed. Wilson G. S., Miles A. A. London: Edward Arnold and Co;
  15. Vonwiller P. 1927; Microscopy with incident light and its application to living objects. J. R. micr. Soc 3 47:325
    [Google Scholar]
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