SUMMARY: A method is described for the examination of growing micro-organisms by vertical and oblique incident illumination using an illuminator of the ‘Universal Illuminator’ (Messrs Cooke, Troughton and Simms) type. A moist chamber has been developed to enable this method to be used for the routine examination of bacterial cultures, and examples are given of both aerobic and anaerobic cultures on the surface of solid nutrient media, preferably containing nigrosine.

This method has also been used for following spore germination of in a liquid medium. A method using Cellophane as a support for growth is also described. Certain organisms can be grown on Cellophane ‘windows’ made in the caps of Bijou bottles containing a liquid medium; this apparatus is suitable for the repeated but not continuous examination of young growths.


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