1887

Abstract

Mice were inoculated intracerebrally with mixtures of the influenza virus strain NWS and the serologically distinct non-neurotropie strains MEL, OcI and SW. From mouse brains removed about the 5th or 6th day virus strains were obtained which, after at least two passages at limiting infective dilution, showed active encephalitogenic power with the serological character of the second strain of the original mixture. These are regarded as recombinants. The strains in general show characters close to those of the serologically similar ‘parent’. They have, however, diminished enzymie action on ovomucin and do not readily clute from red cells; in this they resemble the strain NWS. They are more readily converted to the indicator state than either of the original strains.

The process by which these unusual types arise is discussed in the light of recent views that intracellular multiplication of bacterial viruses and of some animaal viruses takes place not by binary fission but by the breaking down of the infective unit into smaller particles which are the replicating units. It is concluded that the recombinants arise by recenstitution of infective particles from the pool of repticating units produced by double infection of a single cell by virus particles of both parent types.

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/content/journal/micro/10.1099/00221287-5-1-67
1951-02-01
2022-01-18
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References

  1. Avery O. T., Mcleod C. M., Mccarty M. 1944; Studies on the chemical nature of the substance inducing transformation of pneumococcal types. . J. exp. Med 79:137
    [Google Scholar]
  2. Bauer D. J. 1949; Multiplication of the animal viruses. Nature; Lond: 164:767
    [Google Scholar]
  3. Burnet F. M. 1949; Ovomucin as a substrate for the mucinolytic enzymes of V. choleraefiltrates. . Aust. J. exp. Biol. med. Sci 27:245
    [Google Scholar]
  4. Burnet F. M. 1951a; A genetic approach to variation in influenza viruses. 1. The characters of three substrains of influenza virus A (WS). J. gen. Microbiol. 5:46
    [Google Scholar]
  5. Burnet F. M. 1951b; .b. A genetic approach to variation in influenza viruses. 2. Variation in the strain NWS on allantoic passage. J. gen. Microbiol 5:54
    [Google Scholar]
  6. Burnet F. M., Lind P. E. 1951; A genetic approach to variation in influenza viruses. 3. Recombination of characters in influenza virus strains used in mixed infections. . J. gen. Microbiol 5:59
    [Google Scholar]
  7. Chu C. M., Dawson I. M., Elford W. J. 1949; Filamentous forms associated with newly-isolated influenza virus. Lancet i:602
    [Google Scholar]
  8. Delbruck M. 1949; Genetique du Bacteriophage. Colloques Internationaux du Centre National de la Recherche Scientifique, 8:91
    [Google Scholar]
  9. Delbruck M., Bailey W. T. 1946; . Induced mutations in bacterial viruses. Cold Spr. Harb. . . Symp. quant. Biol 11:55
    [Google Scholar]
  10. Gottschalk A., Lind P. E. 1949; Ovomucin, a substrate for the enzyme of influenza virus. 1. Ovomucin as an inhibitor of haemagglutination by heated LEE virus. . Brit. J. exp. Path 30:85
    [Google Scholar]
  11. Henle W., Henle G. 1949; Studies on host-virus interactions in the chick embryo-influenza virus system. III. Development of infectivity, haemagglutination and complement fixation activities during the first infectious cycle. . J. exp. Med 90: 23
    [Google Scholar]
  12. Hershey A. D., Rotman R. 1948; Linkage among genes controlling inhibition of lysis in a bacterial virus. Proc. nat. Acad. Sci., Wash 34:89
    [Google Scholar]
  13. Hoyle L. 1948; The growth cycle of influenza virus A. A study of the relations between virus, soluble antigen and host cell in fertile eggs inoculated with influenza virus. . Brit. J. exp. Path 29:390
    [Google Scholar]
  14. Isaacs A., Edney M. E. 1951; Interference between inactive and active influenza viruses in the chick embryo. IV. The early stages of virus multiplication and interference. . Aust. J. exp. Biol. med. Sci (In the Press.)
    [Google Scholar]
  15. Isaacs A., Edney M. E., Donnelley M., Ingram M. W. 1950; Influenza in an isolated community. An epidemic on Ocean Island. Lancet, i:64
    [Google Scholar]
  16. Luria S. E. 1947; Reactivation of irradiated bacteriophage by transfer of self- reproducing units. . Proc. nat. Acad. Sci., Wash 33:253
    [Google Scholar]
  17. Stone J. D. 1949; . Inhibition of influenza virus haemagglutination by mucoids. I. Conversion of virus to indicator for inhibition. Aust. J. exp. Biol. med. Sci 27:337
    [Google Scholar]
  18. Tatum E. L., Lederberg J. 1947; Gene recombination in the bacterium Escherichia coli, . J. Bact 53:673
    [Google Scholar]
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