1887

Abstract

SUMMARY: Of fifty strains of from human infections, seven of the ‘vulgaris’ and forty-three of the ‘mirabilis’ fermentative type, forty-four were brought into Belyavin’s phase A. which represents the modal S form of recently-isolated strains, and analysed serologically by their H and O antigens. Three of the six remaining strains were irreversibly R. and three were too unstable antigemcally for the routine analysis.

The antigens determining the somatic O types were resistant to heating at 95°, to ethanol and to dilute HCl, treatments that destroyed the H antigens. Serologically similar O antigens were extracted from the bacilli by hot formamide and by ethanol precipitation of tryptie digests.

As in other recent surveys of Proteus serology, the classification of strains by major O antigens was more clear-cut than by H antigens, though some minor O antigens were distributed differently from the major. The forty-four phase-A strains fell into eighteen O-antigenic types, three of these being the classical OX2, OX19 and OXK types, strains of which were included in the survey. The H antigens of each strain were often apparently multiple. but grouping by major H antigens was broader than by O antigens; moreover. H antigens were often common to strains with dissimilar O antigens.

Apart from the antigenic changes accompanying variation from phase A to phases B or C, there were suggestions of other phasic variations in both H and O antigens, which, if substantiated, may necessitate a reconsideration of serological classifications of the group. The three unstable strains in the collection could not be definitely assigned to any O-antigenie type.

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1951-02-01
2022-01-17
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References

  1. Belyavin G. 1951; Cultural and serological phases of Proteus vulgaris. . J. gen. Microbiol 5:197
    [Google Scholar]
  2. Bengston I. A. 1919; The Proteus group of organisms with special reference to agglutination and fermentation reactions and to classification. J. infect. Dis 24:428
    [Google Scholar]
  3. Bergey’s Manual of Determinative Bacteriology 1948, 6th ed.. Breed R. S., Murray E. G. D., Hitchens A. P. editor London: Bailliere, Tindall and Cox;
  4. Cantu CH. 1911; Le bacillus proteus, sa distribution dans la nature. Ann. Inst. Past eur:25852
    [Google Scholar]
  5. Colquhoun D. B., Kirkpatrick J. 1932; The isolation of motile organisms from apparently non-motile cultures of B. typhosus, B. proteus, B. pestis and B. melitensis. . J. Path. Bact 35:367
    [Google Scholar]
  6. Dreyer G., Inman A. C. 1917; The agglutination curve and its importance in the diagnosis of typhoid and paratyphoid fevers in inoculated persons. Lancet i:365
    [Google Scholar]
  7. Duncan J. T. 1935; Inactivation of the ‘H’ antigen by dilute mineral acid. Brit. J. exp. Path 16:405
    [Google Scholar]
  8. Felix A. 1923; Über Varianten der ProteusäStamme. Z. ImmunForsch 35:57
    [Google Scholar]
  9. Fletcher W., Lesslar J. E. 1926; The Weil-Felix reaction in sporadic tropical typhus. Bull. Inst. med. Res. F.M.S1
    [Google Scholar]
  10. Fuller A. T. 1938; The formamide method for the extraction of polysaccharides from haemolytic streptococci. Brit. J. exp. Path 19:130
    [Google Scholar]
  11. Gladstone G. P., Fildes P. 1940; A simple culture medium for general use without meat extract or peptone. Brit. J. exp. Path 21:161
    [Google Scholar]
  12. Hemmes L. 1948 Bijdrage tot der kennis van het geslacht Proteus Wageninjen: Vienman and Zoren;
    [Google Scholar]
  13. Horowitz A. 1916; Contribution à 1’étude du genre Proteus vulgaris. . Ann. Inst. Pasteur 30:307
    [Google Scholar]
  14. Kauffmann F., Perch B. 1947; On the occurrence of Proteus×strains in Denmark. Acta path, microbiol. scand 24:135
    [Google Scholar]
  15. Klieneberger C. 1908; Klinische und kritische Beiträge zur Differenzierung pathogenen Proteus Arten und Beiträge zur Wertung der Proteus Agglutination. Z. Hyg. InfektKr 58:85
    [Google Scholar]
  16. Loghem J.J.Van, Loghem Pouw J.G.W.Van. 1912; Beitragzur Differenzierung der Proteus Gruppe. (B. proteus anindologenes). . Zbl. Bakt 1 Abt. 66:19
    [Google Scholar]
  17. Miles A. A. 1951; The mouse-virulence and toxicity of Proteus vulgaris. . J. gen. Microbiol 5: In the Press
    [Google Scholar]
  18. Moltke O. 1927 Contributions to the characterization and systematic classification of B. proteus vulgaris Hauser Copenhagen: Levin and Munksgaard;
    [Google Scholar]
  19. Pampana E. J. 1933; Microbic dissociation: detection of the ‘R’ variant by means of a specific drop agglutination. J. Hyg., Camb 33:402
    [Google Scholar]
  20. Pandit S. R., Wilson G. S. 1932; The relation between specific and non-specific agglutination in the Brucella group. J. Hyg., Corrib 32:45
    [Google Scholar]
  21. Perch B. 1948; On the serology of the Proteus group. Acta path, microbiol. scand 25:703
    [Google Scholar]
  22. Rustigian R., Stuart C. A. 1945; Biochemical and serological relationships of organisms of genus Proteus. . J. Bact 49:419
    [Google Scholar]
  23. Stamp LORD. 1947; The preservation of bacteria by drying. J. gen. Microbiol 1:251
    [Google Scholar]
  24. Taylor J. F. 1928; B. proteus infections. J. Path. Bact 31:897
    [Google Scholar]
  25. Varela G., Zozaya J., Imaz F. 1944; Structural and serological analysis of intestinal group of proteus. Rev. d. Inst, salub. y enferm. trop 5:15
    [Google Scholar]
  26. Weil E. 1920; Über Varianten des Stammes X19. Wien. klin. Wschr 33:61
    [Google Scholar]
  27. Weil E. 1923; Variationsuntersuchungen bei X19. Z. ImmunForsch 35:25
    [Google Scholar]
  28. Weil E., Felix A. 1916; Zur serologischen Diagnose des Fleckfieber. Wien, klin. Wschr 29:33
    [Google Scholar]
  29. Weil E., Felix A. 1917; Weitere Untersuchungen fiber das wesen Fleckfieber- agglutination. Wien. klin. Wschr 30:1509
    [Google Scholar]
  30. Wenner J. J., Rettger L. F. 1919; A systematic study of the Proteus group of bacteria. J. Bact 4:331
    [Google Scholar]
  31. Winkle S. 1943; Untersuchungen zur Weil-Felix reaktion. Zbl. Bakt 1 Abt. 151:3
    [Google Scholar]
  32. Winkle S. 1944; Zur Typendifferenzierung in der Gattung Proteus Hauser. Zbl. Bakt 1 Abt. 151:494
    [Google Scholar]
  33. Wolf S. 1899; Beiträge zur Lehre der Agglutination mit besonderer Bezugnahme auf die Differenzierung der Coli- und Proteus-gruppe und auf die Mischinfektion. Zbl. Bakt 1 Abt. 25:311
    [Google Scholar]
  34. Yacob M. 1932; Studies of genus Proteus. . Indian J. med. Res 19:787
    [Google Scholar]
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