1887

Abstract

SUMMARY

The lytic action of lysozyme upon walls was studied by following the disappearance of bacillary-colony-forming units and the appearance of L-colony-forming-units. The rapidity of cell wall removal by lysozyme fluctuated markedly during growth in a chemically defined medium, presumably because subtle changes in the cell wall were constantly occurring. When lysozyme-sensitive bacilli were grown with chloram-phenicol 10 g./ml. for 3 hr they showed a notable increase in lysozyme resistance; at the same time, their walls almost doubled in thickness. As lysozyme attack proceeded in a given culture, the bacilli passed first through a rod-shaped osmotically sensitive stage, and then a spherical stage characterized by incomplete removal of cell wall before finally reaching the naked protoplast stage. The spherical forms with adherent wall residues formed L colonies on a medium containing the reversion inhibitor D-methionine and bacillary colonies on the same medium without D-methionine. Under the latter conditions, the cell wall residue served as a starting point for rebuilding of complete wall, much as residual wall permits reversion of Gram-negative spheroplasts to the bacillary state. In the presence of methionine, the feedback sequence required for wall formation was severed, resulting in heritable propagation of the protoplast state.

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1967-12-01
2024-04-20
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References

  1. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. J. Bact 81:741
    [Google Scholar]
  2. Archer L. J., Landman O. E. 1967; Transformation of unlinked loci in synchronized Bacillus subtilis cultures with chromosomes arrested at the origin. Bact. Proc52
    [Google Scholar]
  3. Charpak M., Dedonder R. 1965; Production d’un ‘facteur de competence’ soluble par B. subtilis Marburg ind-168. C. r. hebd. Séanc. Acad. Sci., Paris 260:5638
    [Google Scholar]
  4. Chung K. L., Hawirko R. Z., Isaac P. K. 1964; Cell wall replication. I. Cell wall growth of B. cereus and B. megaterium. Can. J. Microbiol 10:43
    [Google Scholar]
  5. Cole R. M. 1965; Bacterial cell wall replication followed by immunofluorescence. Bact. Rev 29:326
    [Google Scholar]
  6. Fitz-James P. C. 1967; The isolation of mesosomal vesicles extruded during protoplasting. In Microbiol Protoplasts, Spheroplasts and L-Forms Guze L. B. Baltimore: Williams and Wilkins;
    [Google Scholar]
  7. Hancock R. 1960; Accumulation of pool amino acids in Staphylococcus aureus following inhibition of protein synthesis. Biochim. biophys. Acta 37:47
    [Google Scholar]
  8. Hancock R., Park J. T. 1958; Cell wall synthesis by S. aureus in the presence of chloram- phenicol. Nature, Lond 181:1050
    [Google Scholar]
  9. Hash J. H., Davies M. C. 1962; Electron microscopy of Staphylococcus aureus treated with tetracycline. Science, N. Y 138:828
    [Google Scholar]
  10. Jensen R. H., Haas F. 1963; Electrokinetics and cell physiology. II. Relationship of surface change to onset of bacterial competence for genetic transformation. J. Bact 86:79
    [Google Scholar]
  11. Kellenberger E., Ryter A., Séchaud J. 1958; Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. biophys. biochem. Cytol 4:671
    [Google Scholar]
  12. King J. R., Gooder H. 1965; Subsequent growth as l-forms or streptococci of lysozyme- damaged group D streptococci. Bact. Proc58
    [Google Scholar]
  13. Landman O. E. 1967; Protoplasts, spheroplasts and l-forms viewed as a genetic system. In Microbiol Protoplasts, Spheroplasts and L-Forms Guze L. G. Baltimore: Williams and Wilkins;
    [Google Scholar]
  14. Landman O. E., Ginoza H. S. 1961; Genetic nature of stable l-forms of Salmonella paratyphi. J. Bact 81:875
    [Google Scholar]
  15. Landman O. E., Halle S. 1963; Enzymically and physically induced inheritance changes in Bacillus subtilis. J. molec. Biol 7:721
    [Google Scholar]
  16. Landman O. E., Altenbern R. A., Ginoza H. S. 1958; Quantitative conversion of cells and protoplasts of Proteus mirabilis and Escherichia coli to the l-form. J. Bact 75:567
    [Google Scholar]
  17. Luft J. H. 1961; Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol 9:409
    [Google Scholar]
  18. Martin H. H. 1966; Biochemistry of bacterial cell walls. A. Rev. Biochem 35:457
    [Google Scholar]
  19. Miller I. L., Landman O. E. 1965; The effect of chloramphenicol pretreatment of Bacillus subtilis on its cell wall and lysozyme spheres. Bad. Proc27
    [Google Scholar]
  20. Newton B. A. 1965; Mechanism of antibiotic action. A. Rev. Microbiol 19:209
    [Google Scholar]
  21. Pakula R., Walczac W. 1963; On the nature of competence of transformable Streptococci. J. gen. Microbiol 31:125
    [Google Scholar]
  22. Ryter A., Landman O. E. 1964; Electron microscope study of the relationship between mesosome loss and the stable l-state (or protoplast state) in Bacillus subtilis. J. Bact 88:457
    [Google Scholar]
  23. Ryter A., Landman O. E. 1967; Morphological study of the attachment of nucleoid to membrane in bacilli, protoplasts and reverting protoplasts of Bacillus subtilis. In Microbiol Protoplasts, Spheroplasts and L-Forms Guze B. Baltimore: Williams and Wilkins;
    [Google Scholar]
  24. Salton M. R. J. 1964 The Bacterial Cell Wall Amsterdam: Elsevier;
    [Google Scholar]
  25. Shockman G. D. 1965; Unbalanced cell wall synthesis: autolysis and cell-wall thickness. Bact. Rev 29:345
    [Google Scholar]
  26. Shockman G. D. 1967; The relationship of autolysin to lysozyme sensitivity of Streptococcus faecalis. In Microbial Protoplasts, Spheroplasts and L-Forms Guze B. L. Baltimore: Williams and Wilkins;
    [Google Scholar]
  27. Tomasz A., Beiser S. M. 1965; Relationship between the competence antigen and the competence-activator substance in Pneumococci. J. Bact 90:1226
    [Google Scholar]
  28. Tomasz A., Hotchkiss R. D. 1964; Regulation of the transformability of Pneumococcal cultures by macromolecular cell products. Proc. natn. Acad. SciU.S.A 51480
    [Google Scholar]
  29. Toennies G., Bakay B., Shockman G. D. 1955; Bacterial composition and growth phase. J. biol. Chem 234:3269
    [Google Scholar]
  30. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia coli strain b. J. gen. Microbiol 22:158
    [Google Scholar]
  31. Whitney J. G., Grula E. A. 1964; Incorporation of d-serine into the cell wall mucopeptide of Micrococcus lysodeikticus. Biochem. biophys. Res. Comm 14:375
    [Google Scholar]
  32. Young F. E. 1965; Variation in the chemical composition of the cell walls of Bacillus subtilis during growth in different media. Nature, Lond 207:104
    [Google Scholar]
  33. Young F. E. 1966; Autolytic enzyme associated with cell walls of Bacillus subtilis. J. biol. Chem 241:3462
    [Google Scholar]
  34. Young F. E. 1967; Competence in Bacillus subtilis transformation system. Nature, Lond 213:773
    [Google Scholar]
  35. Young F. E., Spizizen J., Crawford I. P. 1963; Biochemical aspects of competence in the Bacillus subtilis transformation system. I. Chemical composition of cell walls. J. biol. Chem 238:3119
    [Google Scholar]
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