SUMMARY: Material produced by growing and required for the initiation of growth of from small inocula (growth-initiating substance, GIS) was purified by Sephadex gel filtration and ion-exchange resin chromatography. The purified material was characterized as an anionic compound of low molecular weight, heat-stable at neutral pH and in dilute alkali but destroyed by heating in dilute acid. Out of large variety of known nutrients tested, only iron salts and some iron-chelating compounds (sider-amines) replaced partially GIS in supporting growth of different strains from small inocula. GIS formed complexes with iron and copper ions. The production of GIS by in a chemically defined medium was enhanced by added ornithine. Isotopic evidence established the role of ornithine as a biosynthetic precursor of GIS.


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